Sensory Metabolite Profiling in a Date Pit Based Coffee Substitute and in Response to Roasting as Analyzed via Mass Spectrometry Based Metabolomics
| Autor: | Shaimaa A. ElShebiney, Asmaa Otify, Ludger A. Wessjohann, Mohamed A. Farag, Camilia G. Michel, Aly M. El-Sayed, Anja Ehrlich |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Phoenix dactylifera Metabolite Phytochemicals coffee Pharmaceutical Science 01 natural sciences Article Mass Spectrometry Analytical Chemistry Beverages lcsh:QD241-441 chemistry.chemical_compound Mice 0404 agricultural biotechnology Metabolomics lcsh:Organic chemistry Drug Discovery Metabolome Animals Food science Physical and Theoretical Chemistry Roasting date palm Volatile Organic Compounds Chemistry 010401 analytical chemistry Organic Chemistry Primary metabolite food and beverages 04 agricultural and veterinary sciences 040401 food science Terpenoid 0104 chemical sciences volatiles multivariate analysis Chemistry (miscellaneous) Molecular Medicine Caffeine |
| Zdroj: | Molecules Volume 24 Issue 18 Molecules, Vol 24, Iss 18, p 3377 (2019) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules24183377 |
| Popis: | Interest in developing coffee substitutes is on the rise, to minimizing its health side effects. In the Middle East, date palm (Phoenix dactylifera L.) pits are often used as a coffee substitute post roasting. In this study, commercially-roasted date pit products, along with unroasted and home-prepared roasted date pits, were subjected to analyses for their metabolite composition, and neuropharmacological evaluation in mice. Headspace SPME-GCMS and GCMS post silylation were employed for characterizing its volatile and non-volatile metabolite profile. For comparison to roasted coffee, coffee product was also included. There is evidence that some commercial date pit products appear to contain undeclared additives. SPME headspace analysis revealed the abundance of furans, pyrans, terpenoids and sulfur compounds in roasted date pits, whereas pyrroles and caffeine were absent. GCMS-post silylation employed for primary metabolite profiling revealed fatty acids&rsquo enrichment in roasted pits versus sugars&rsquo abundance in coffee. Biological investigations affirmed that date pit showed safer margin than coffee from its LD50, albeit it exhibits no CNS stimulant properties. This study provides the first insight into the roasting impact on the date pit through its metabolome and its neuropharmacological aspects to rationalize its use as a coffee substitute. |
| Databáze: | OpenAIRE |
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