Preparation of Induced Pluripotent Stem Cells Using Human Peripheral Blood Monocytes
| Autor: | Masashi Kondo, Sumito Isogai, Kazuyoshi Imaizumi, Naoki Yamamoto, Yasuhiro Goto, Masamichi Hayashi, Noriko Hiramatsu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
three germ layers induced pluripotent stem cells medicine.medical_treatment Genetic Vectors Cell Cell Culture Techniques human monocytes Germ layer Biology self-renewal Sendai virus Monocytes Mice 03 medical and health sciences Immune system Antigen medicine Animals Humans Induced pluripotent stem cell Research Articles Cell Biology Cell biology Transplantation 030104 developmental biology Cytokine medicine.anatomical_structure Cell culture Developmental Biology Biotechnology |
| Zdroj: | Cellular Reprogramming |
| ISSN: | 2152-4998 2152-4971 |
| DOI: | 10.1089/cell.2018.0024 |
| Popis: | Since induced pluripotent stem (iPS) cells have been established, in recent years, clinical transplantation of cells differentiated from iPS cells derived from human skin fibroblasts is been in progress. On the contrary, monocytes have complete genome information without damage and gene recombination, they are contained in the peripheral blood by ∼3%–8% and differentiate into dendritic cells that are the type of control tower for immune cells. However, generation of monocyte-derived iPS cells has only been successful when special persistent Sendai virus vectors have been used. Therefore, in this study, as a preculture method for monocytes, a culture method for maintaining activity without using any cytokine was established, and using a commercially available vector without genetic toxicity without damaging the chromosome of the cell, iPS cells derived from monocytes were successfully produced. This cell has the ability to differentiate into three germ layers, and when compared with commercially available iPS cells, there was no significant difference between self-renewal and gene expression in the three germ layers. In future, we will compare the differentiation induction of monocyte-derived iPS cells with dendritic cells and investigate the production of dendritic cells that can cope with various antigens. |
| Databáze: | OpenAIRE |
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