Dual-Color Fluorescent Timer Enables Detection of Growth-Arrested Pathogenic Bacterium
| Autor: | Lei Ni, Jundong Han, Aiguo Xia, Fan Jin, Shuai Yang, Zhenyu Jin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Multidrug tolerance biology Pseudomonas aeruginosa Chemistry 030106 microbiology Green Fluorescent Proteins Pathogenic bacteria medicine.disease_cause biology.organism_classification Fluorescence Nutrient starvation Cell biology 03 medical and health sciences Kinetics 030104 developmental biology Infectious Diseases medicine Timer Dual color Bacteria |
| Zdroj: | ACS infectious diseases. 4(12) |
| ISSN: | 2373-8227 |
| Popis: | We present a method capable of detecting single slow-growing and growth-arrested cells in a bacterial culture composed of physiologically and phenotypically different cells. Unlike the use of transcriptional reporters to gauge the metabolic activities in cells, here, we fuse two different fluorescent proteins with distinctive maturation rates to construct a timer to directly determine the growth rate of single Pseudomonas aeruginosa cells. We demonstrate that the dual-color fluorescent timer can indicate the slow-growing and growth-arrested cells from bacterial cultures in the presence of various environmental stresses, including nutrient starvation or antibiotic treatments, which greatly expand the methods for detecting and isolating persister cells. |
| Databáze: | OpenAIRE |
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