Extremely Low-Frequency Electromagnetic Fields Promote In Vitro Neuronal Differentiation and Neurite Outgrowth of Embryonic Neural Stem Cells via Up-Regulating TRPC1

Autor: Gang Zhu, Zhengwang Cao, Shangcheng Xu, Chuan Liu, Chunhai Chen, Zhengping Yu, Liping Pei, Qinlong Ma, Min Li, Mindi He, Yonghui Lu, Weixia Duan, Ping Deng, Min Lin, Yanwen Zhang, Lei Zhang, Min Zhong, Huifeng Pi, Zhou Zhou
Rok vydání: 2015
Předmět:
0301 basic medicine
Cellular differentiation
Organogenesis
lcsh:Medicine
Apoptosis
Mice
0302 clinical medicine
Neural Stem Cells
Animal Cells
RNA
Small Interfering
lcsh:Science
NeuroD
Neurons
Staining
Mice
Inbred BALB C
Multidisciplinary
Cell Death
Neurogenesis
Brain
Cell Staining
Cell Differentiation
respiratory system
Neural stem cell
Cell biology
Up-Regulation
Cell Processes
Biological Cultures
Cellular Types
Neuronal Differentiation
Research Article
animal structures
Neurite
Proneural genes
Biology
Research and Analysis Methods
03 medical and health sciences
Electromagnetic Fields
Neurosphere
Neurites
Animals
Embryonic Stem Cells
Cell Proliferation
TRPC Cation Channels
lcsh:R
Brain Development
Biology and Life Sciences
Cell Biology
Neuronal Dendrites
Cell Cultures
Embryonic stem cell
030104 developmental biology
Specimen Preparation and Treatment
lcsh:Q
Neurospheres
Organism Development
030217 neurology & neurosurgery
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 3, p e0150923 (2016)
ISSN: 1932-6203
Popis: Exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) can enhance hippocampal neurogenesis in adult mice. However, little is focused on the effects of ELF-EMFs on embryonic neurogenesis. Here, we studied the potential effects of ELF-EMFs on embryonic neural stem cells (eNSCs). We exposed eNSCs to ELF-EMF (50 Hz, 1 mT) for 1, 2, and 3 days with 4 hours per day. We found that eNSC proliferation and maintenance were significantly enhanced after ELF-EMF exposure in proliferation medium. ELF-EMF exposure increased the ratio of differentiated neurons and promoted the neurite outgrowth of eNSC-derived neurons without influencing astrocyes differentiation and the cell apoptosis. In addition, the expression of the proneural genes, NeuroD and Ngn1, which are crucial for neuronal differentiation and neurite outgrowth, was increased after ELF-EMF exposure. Moreover, the expression of transient receptor potential canonical 1 (TRPC1) was significantly up-regulated accompanied by increased the peak amplitude of intracellular calcium level induced by ELF-EMF. Furthermore, silencing TRPC1 expression eliminated the up-regulation of the proneural genes and the promotion of neuronal differentiation and neurite outgrowth induced by ELF-EMF. These results suggest that ELF-EMF exposure promotes the neuronal differentiation and neurite outgrowth of eNSCs via up-regulation the expression of TRPC1 and proneural genes (NeuroD and Ngn1). These findings also provide new insights in understanding the effects of ELF-EMF exposure on embryonic brain development.
Databáze: OpenAIRE
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