Neurofilaments from ox spinal nerves. Isolation, disassembly, reassembly and cross-linking properties
Autor: | Martin J. Carden, P A M Eagles |
---|---|
Rok vydání: | 1983 |
Předmět: |
Male
Neurofilament Macromolecular Substances Cell Fractionation Biochemistry Protein filament Intermediate Filament Proteins Neurofilament Proteins Animals Disulfides Molecular Biology Cytoskeleton chemistry.chemical_classification Molecular mass Chemistry food and beverages Cell Biology Molecular Weight Microscopy Electron Cross-Linking Reagents Spinal Nerves Thiol Cattle Electrophoresis Polyacrylamide Gel Phenanthrolines Research Article |
Zdroj: | The Biochemical journal. 215(2) |
ISSN: | 0264-6021 |
Popis: | An isolation procedure for neurofilaments from ox spinal nerves is described where the triplet polypeptides (which have molecular weights of 205 000, 158 000 and 72 000) constitute more than 80% of the preparation. Soon after purification, the neurofilaments form a gel that is stable for many weeks. The purified neurofilaments disassemble in low-salt buffers at pH greater than 7.0 into soluble particles that contain all of the triplet polypeptides. Greater than 90% of the protein can reassemble to form filaments. The thiol-containing residues in the filaments can be cross-linked. Analyses of the complexes formed show that in the filament the 205 000-mol.wt. components are arranged to that they can be cross-linked to themselves and to the 158 000-mol.wt. polypeptides, and that the 72 000-mol.wt. components are arranged so that their thiol groups can be cross-linked together. |
Databáze: | OpenAIRE |
Externí odkaz: |