Theosr1andosr2genes act in the pronephric anlage downstream of retinoic acid signaling and upstream ofwnt2bto maintain pectoral fin development
| Autor: | José Luis Gómez-Skarmeta, Ana Neto, Nadia Mercader |
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| Přispěvatelé: | Junta de Andalucía, Fundação para a Ciência e a Tecnologia (Portugal), Fundación Pro CNIC, Universidade do Porto (Portugal) |
| Rok vydání: | 2012 |
| Předmět: |
Limb Buds
Retinoic acid Limb bud formation Tretinoin 610 Medicine & health Real-Time Polymerase Chain Reaction Limb Pronephros Wnt2 Protein Mesoderm chemistry.chemical_compound Limb bud tbx5a Osr genes Forelimb Animals Dimethyl Sulfoxide Molecular Biology Zebrafish In Situ Hybridization DNA Primers Genetics biology Lateral plate mesoderm Wnt signaling pathway Morphant wnt2b Zebrafish Proteins biology.organism_classification Cell biology chemistry p-Aminoazobenzene Animal Fins T-Box Domain Proteins Intermediate mesoderm Signal Transduction Transcription Factors Developmental Biology |
| Zdroj: | Neto, Ana; Mercader Huber, Nadia; Gómez-Skarmeta, José Luis (2012). The Osr1 and Osr2 genes act in the pronephric anlage downstream of retinoic acid signaling and upstream of Wnt2b to maintain pectoral fin development. Development, 139(2), pp. 301-311. Company of Biologists Limited 10.1242/dev.074856 Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.074856 |
| Popis: | Vertebrate odd-skipped related genes (Osr) have an essential function during the formation of the intermediate mesoderm (IM) and the kidney structures derived from it. Here, we show that these genes are also crucial for limb bud formation in the adjacent lateral plate mesoderm (LPM). Reduction of zebrafish Osr function impairs fin development by the failure of tbx5a maintenance in the developing pectoral fin bud. Osr morphant embryos show reduced wnt2b expression, and increasing Wnt signaling in Osr morphant embryos partially rescues tbx5a expression. Thus, Osr genes control limb bud development in a non-cell-autonomous manner, probably through the activation of Wnt2b. Finally, we demonstrate that Osr genes are downstream targets of retinoic acid (RA) signaling. Therefore, Osr genes act as a relay within the genetic cascade of fin bud formation: by controlling the expression of the signaling molecule Wnt2ba in the IM they play an essential function transmitting the RA signaling originated in the somites to the LPM. We thank the Spanish and Andalusian Governments [grants BFU2010-14839, CSD2007-00008, BFU2008-00212/BMC and RYC-2006-001694], the Proyecto de Excelencia [grant CVI-3488] and the Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III ProCNIC. A.N. is funded by Fundaçao para a Ciencia e Tecnologia from Portugal [grant SFRH/BD/15242/2004] and belongs to the Graduate Program in Areas of Basic and Applied Biology from Oporto University. |
| Databáze: | OpenAIRE |
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