Molecular characterization and in situ mRNA localization of the neural recognition molecule J1-160/180: a modular structure similar to tenascin
| Autor: | E S Wintergerst, Udo Bartsch, Melitta Schachner, B Fuss |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cell Adhesion Molecules
Neuronal Molecular Sequence Data Tenascin Nerve Tissue Proteins Polymerase Chain Reaction Tenascin X Cell Adhesion Animals Tenascin-R Tissue Distribution RNA Messenger Cloning Molecular Peptide sequence In Situ Hybridization Extracellular Matrix Proteins Messenger RNA Base Sequence biology Alternative splicing Nucleic acid sequence DNA Articles Cell Biology Molecular biology Rats Alternative Splicing Oligodeoxyribonucleotides Tenascin Family biology.protein |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.120.5.1237 |
| Popis: | The oligodendrocyte-derived extracellular matrix glycoprotein J1-160/180 is a recognition molecule expressed exclusively in the central nervous system. J1-160/180 has been shown to be adhesive for astrocytes and repellent towards neurons and growth cones. We report here the complete nucleotide sequence of J1-160/180 in the rat. The predicted amino acid sequence showed a structural architecture very similar to tenascin: a cysteine-rich amino terminal region is followed by 4.5 epidermal growth factor-like repeats, 9 fibronectin type III homologous repeats and a domain homologous to fibrinogen. Sequence comparison analysis revealed highest homology of rat J1-160/180 to mouse tenascin and chicken restrictin with a similarity of 66% and 85%, respectively. The J1-160/180-coding mRNA is derived from a single copy gene. Using the polymerase chain reaction we could show that two J1-160/180 isoforms are generated by alternative splicing of the sixth fibronectin type III homologous repeat. Localization of J1-160/180 mRNA by in situ hybridization in the cerebellum, hippocampus and olfactory bulb confirmed the expression of J1-160/180 by oligodendrocytes with a peak of transcription at 7-14 d after birth, indicating a functional role during myelination. In addition, J1-160/180-specific RNA was found in a small subset of neurons in all three structures of the CNS analyzed. These neurons continue to express J1-160/180 in the adult. |
| Databáze: | OpenAIRE |
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