Variant histone H3.3 is deposited at sites of nucleosomal displacement throughout transcribed genes while active histone modifications show a promoter-proximal bias
| Autor: | Oliver Bell, Christiane Wirbelauer, Dirk Schübeler |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Fluorescent Antibody Technique
Biology Molecular biology Research Communications Nucleosomes Cell biology Histones Kinetics Histone H3 Histone H1 Histone methyltransferase Histone methylation Histone H2A Genetics Animals Histone code Nucleosome Drosophila Histone octamer Promoter Regions Genetic Developmental Biology |
| Zdroj: | Genes & Development. 19:1761-1766 |
| ISSN: | 1549-5477 0890-9369 |
| Popis: | Deposition of variant histones provides a mechanism to reset and to potentially specify chromatin states. We determined the distribution of H3 and its variant H3.3 relative to chromatin structure and elongating polymerase. H3.3 is enriched throughout active genes similar to polymerase, yet its distribution is very distinct from that of several euchromatic histone modifications, which are highly biased toward the 5′ part of active genes. Upon gene induction we observe displacement of both H3 and H3.3 followed by selective deposition of H3.3. These results support a model in which H3.3 deposition compensates for transcription-coupled nucleosomal displacement yet does not predetermine tail modifications. |
| Databáze: | OpenAIRE |
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