Determination of Aniline, 4-Aminoazobenzene, and 2-Naphthol in the Color Additive D&C Red No. 17 Using Ultra-High-Performance Liquid Chromatography
| Autor: | H.-H. Wendy Yang, Adrian Weisz |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Acetonitriles
Calibration curve Food Contamination Naphthols Acetates 010501 environmental sciences 01 natural sciences Analytical Chemistry chemistry.chemical_compound Column chromatography Aniline Limit of Detection Environmental Chemistry Acetonitrile Chromatography High Pressure Liquid 0105 earth and related environmental sciences Pharmacology Detection limit Aniline Compounds Chromatography 010401 analytical chemistry Food Coloring Agents 0104 chemical sciences chemistry p-Aminoazobenzene Calibration Particle size Azo Compounds Agronomy and Crop Science Ammonium acetate 2-Naphthol Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 101:1961-1966 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.5740/jaoacint.17-0502 |
| Popis: | Specifications in the U.S. Code of Federal Regulations for the color additive D&C Red No. 17 (R17, Colour Index No. 26100) limit the levels of the dye’s intermediates, aniline (AN), 2-naphthol (β-naphthol, BN), and 4-aminoazobenzene (4AAB), to 0.2, 0.2, and 0.1%, respectively. The present work reports the development and application of an ultra-HPLC method for the quantitative determination of these impurities in R17. A 1.7 μm particle size C-18 column was used with 0.2 M ammonium acetate and acetonitrile as the eluents. AN, BN, and 4AAB were quantified by using six-point calibration curves with data points (w/w) ranging from 0.01 to 0.25% for AN, 0.01 to 0.24% for BN, and 0.01 to 0.19% for 4AAB. The correlation coefficients ranged from 0.9992 to 0.9999. Limits of detection for the analytes ranged from 0.002 to 0.01%. Recoveries of the analytes ranged from 99.5 to 102%. Relative standard deviations ranged from 0.482 to 1.262%. The new method was applied to analyze portions from 22 batches of R17 submitted to the U.S. Food and Drug Administration for certification. It was found to be simpler to implement, faster, and more sensitive than the older gravity-elution column chromatography method, which it has replaced. |
| Databáze: | OpenAIRE |
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