Functionalized rare earth-doped nanoparticles for breast cancer nanodiagnostic using fluorescence and CT imaging
| Autor: | Fernando M. Guerra-Olvera, Vladimir Mendoza-Lavaniegos, Gustavo A. Hirata, Enrique Iñiguez, Thomas Kretzschmar, Patricia Juárez, Akhil Jain, Pierrick G.J. Fournier, Prakhar Sengar |
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| Rok vydání: | 2018 |
| Předmět: |
Pharmaceutical Science
Medicine (miscellaneous) Gadolinium 02 engineering and technology 01 natural sciences Applied Microbiology and Biotechnology Metastasis Mice Breast cancer Cytotoxic T cell Internalization media_common Folate receptor medicine.diagnostic_test Chemistry 021001 nanoscience & nanotechnology lcsh:R855-855.5 Injections Intravenous Heterografts Molecular Medicine Female 0210 nano-technology lcsh:Medical technology lcsh:Biotechnology media_common.quotation_subject Biomedical Engineering Breast Neoplasms Bioengineering 010402 general chemistry Folic Acid Europium In vivo lcsh:TP248.13-248.65 Cell Line Tumor medicine Animals Humans Mammography Particle Size Luminescent nanoparticles Research Cancer detection medicine.disease Molecular medicine 0104 chemical sciences HEK293 Cells Luminescent Measurements Cancer research Nanoparticles Tomography X-Ray Computed |
| Zdroj: | Journal of Nanobiotechnology Journal of Nanobiotechnology, Vol 16, Iss 1, Pp 1-18 (2018) |
| ISSN: | 1477-3155 |
| DOI: | 10.1186/s12951-018-0359-9 |
| Popis: | Background Breast cancer is the second leading cause of cancer death among women and represents 14% of death in women around the world. The standard diagnosis method for breast tumor is mammography, which is often related with false-negative results leading to therapeutic delays and contributing indirectly to the development of metastasis. Therefore, the development of new tools that can detect breast cancer is an urgent need to reduce mortality in women. Here, we have developed Gd2O3:Eu3+ nanoparticles functionalized with folic acid (FA), for breast cancer detection. Results Gd2O3:Eu3+ nanoparticles were synthesized by sucrose assisted combustion synthesis and functionalized with FA using EDC-NHS coupling. The FA-conjugated Gd2O3:Eu3+ nanoparticles exhibit strong red emission at 613 nm with a quantum yield of ~ 35%. In vitro cytotoxicity studies demonstrated that the nanoparticles had a negligible cytotoxic effect on normal 293T and T-47D breast cancer cells. Cellular uptake analysis showed significantly higher internalization of FA-conjugated RE nanoparticles into T-47D cells (Folrhi) compared to MDA-MB-231 breast cancer cells (Folrlo). In vivo confocal and CT imaging studies indicated that FA-conjugated Gd2O3:Eu3+ nanoparticles accumulated more efficiently in T-47D tumor xenograft compared to the MDA-MB-231 tumor. Moreover, we found that FA-conjugated Gd2O3:Eu3+ nanoparticles were well tolerated at high doses (300 mg/kg) in CD1 mice after an intravenous injection. Thus, FA-conjugated Gd2O3:Eu3+ nanoparticles have great potential to detect breast cancer. Conclusions Our findings provide significant evidence that could permit the future clinical application of FA-conjugated Gd2O3:Eu3+ nanoparticles alone or in combination with the current detection methods to increase its sensitivity and precision. Electronic supplementary material The online version of this article (10.1186/s12951-018-0359-9) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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