a cis antisense RNA operates in trans in S. aureus
| Autor: | Ambre Jousselin, Brice Felden, Nour Sayed |
|---|---|
| Přispěvatelé: | Fonction, structure et inactivation d'ARN bactériens, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) |
| Rok vydání: | 2011 |
| Předmět: |
Erythrocytes
MESH: Sequence Homology Amino Acid MESH: Amino Acid Sequence MESH: Base Sequence Structural Biology MESH: Staphylococcus aureus Protein biosynthesis MESH: RNA Antisense MESH: Bacterial Proteins Peptide sequence Genetics MESH: Gene Expression Regulation Bacterial 0303 health sciences MESH: Peptides MESH: Erythrocytes MESH: Hemolysis Antisense RNA RNA Bacterial [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology MESH: Protein Biosynthesis Transfer RNA MESH: RNA Bacterial Staphylococcus aureus Blotting Western Molecular Sequence Data Biology Hemolysis MESH: Sequence Homology Nucleic Acid 03 medical and health sciences Eukaryotic translation Bacterial Proteins Sequence Homology Nucleic Acid Humans MESH: Blotting Northern MESH: Blotting Western RNA Antisense [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Amino Acid Sequence Molecular Biology Gene 030304 developmental biology MESH: Humans MESH: Molecular Sequence Data Base Sequence Sequence Homology Amino Acid 030306 microbiology RNA Gene Expression Regulation Bacterial Blotting Northern Open reading frame Protein Biosynthesis Peptides |
| Zdroj: | Nature Structural and Molecular Biology Nature Structural and Molecular Biology, Nature Publishing Group, 2012, 19 (1), pp.105-12. ⟨10.1038/nsmb.2193⟩ Nature Structural and Molecular Biology, 2012, 19 (1), pp.105-12. ⟨10.1038/nsmb.2193⟩ |
| ISSN: | 1545-9985 1545-9993 |
| DOI: | 10.1038/nsmb.2193 |
| Popis: | International audience; Antisense RNAs (asRNAs) pair to RNAs expressed from the complementary strand, and their functions are thought to depend on nucleotide overlap with genes on the opposite strand. There is little information on the roles and mechanisms of asRNAs. We show that a cis asRNA acts in trans, using a domain outside its target complementary sequence. SprA1 small regulatory RNA (sRNA) and SprA1(AS) asRNA are concomitantly expressed in S. aureus. SprA1(AS) forms a complex with SprA1, preventing translation of the SprA1-encoded open reading frame by occluding translation initiation signals through pairing interactions. The SprA1 peptide sequence is within two RNA pseudoknots. SprA1(AS) represses production of the SprA1-encoded cytolytic peptide in trans, as its overlapping region is dispensable for regulation. These findings demonstrate that sometimes asRNA functional domains are not their gene-target complementary sequences, suggesting there is a need for mechanistic re-evaluation of asRNAs expressed in prokaryotes and eukaryotes. |
| Databáze: | OpenAIRE |
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