The Evaluation of Human Tenon’s Fibroblasts and Endothelial Cell Responses to Antifibrotics Alone and in Combination with α-Tocopherol
| Autor: | Serap Erdem-Kuruca, Beyza Cetin, Ebru Gurel, Kaya N. Engin, Kadriye Akgün-Dar, Sema Bilgic, Bülent Yemisci, Mehmet Arslan, Sabriye Karadenizli |
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| Rok vydání: | 2014 |
| Předmět: |
Alkylating Agents
Necrosis Paclitaxel Tenon Capsule Mitomycin alpha-Tocopherol Cell Apoptosis Pharmacology Antioxidants Cellular and Molecular Neuroscience chemistry.chemical_compound Human Umbilical Vein Endothelial Cells medicine Humans Fibroblast Cells Cultured bcl-2-Associated X Protein biology Caspase 3 business.industry Cytochrome c Mitomycin C Cytochromes c Fibroblasts Flow Cytometry Sensory Systems Endothelial stem cell Drug Combinations Ophthalmology medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 chemistry Immunology biology.protein Fluorouracil medicine.symptom business |
| Zdroj: | Current Eye Research. 40:19-29 |
| ISSN: | 1460-2202 0271-3683 |
| Popis: | We aimed to evaluate the influence of current antifibrotic agents as well as the possible results obtained by combining these agents. This study included α-tocopherol, a strong antifibrotic and an efficient neuromediator of pathways used by other agents.Mitochondrial Bcl-2, Bax, cytochrome c and cytoplasmic caspase-3 expression, as well as toxic effect patterns, mitosis and cellular reactions due to α-tocopherol alone or combined with paclitaxel, mitomycin C and 5-flurouracil (5-FU), was studied in series obtained from human endothelial and primary Tenon's fibroblast cell cultures.The strongest apoptotic effect in both cell groups belonged to paclitaxel, followed by mitomycin C, and despite the overall suppressive effect of the α-tocopherol combination, mitomycin C increased its efficiency on the endothelial cells. The apoptosis/necrosis ratio was highest in α-tocopherol and lowest in paclitaxel, with α-tocopherol generally decreasing necrosis. Bax was observed at a high level with mitomycin C. Cytotoxicity was the highest with paclitaxel, and the caspase-3 reaction was markedly higher with mitomycin C in both cell types. In the α-tocopherol and 5-FU slides, mitosis and a layered formation were observed. The addition of α-tocopherol reduced the cytotoxicity of all antifibrotic agents in both cell series by decreasing the cell numbers, leading to necrosis.Alone or in combination, the use of α-tocopherol and 5-FU is safer than other agents. By suppressing the cytotoxic effects of other antifibrotic agents, α-tocopherol is a promising drug for improving the effects of antifibrotics in many aspects of medicine. In addition, it has the potential to play a role beyond its antioxidant and antifibrotic activity in ocular surgery. |
| Databáze: | OpenAIRE |
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