Dietary fish oil ameliorates adipose tissue dysfunction in insulin-resistant rats fed a sucrose-rich diet improving oxidative stress, peroxisome proliferator-activated receptor γ and uncoupling protein 2
| Autor: | Yolanda B. Lombardo, Adriana Chicco, Dante Selenscig, María del Rosario Ferreira |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Antioxidant CIENCIAS MÉDICAS Y DE LA SALUD medicine.medical_treatment Peroxisome proliferator-activated receptor Adipose tissue Ciencias de la Salud 030209 endocrinology & metabolism medicine.disease_cause Fat pad 03 medical and health sciences 0302 clinical medicine Insulin resistance Fish Oils Dietary Sucrose Internal medicine medicine Animals Humans Insulin Uncoupling Protein 2 Rats Wistar Dyslipidemias chemistry.chemical_classification FISH OIL Interleukin-6 General Medicine Fish oil medicine.disease SUCROSE-RICH DIET Glutathione Rats Otras Ciencias de la Salud PPAR gamma Oxidative Stress 030104 developmental biology Endocrinology chemistry Adipose Tissue Insulin Resistance ADIPOSE TISSUE DYSFUNCTION Reactive Oxygen Species Corn oil Oxidative stress Food Science INSULIN RESISTANCE |
| Zdroj: | Foodfunction. 9(4) |
| ISSN: | 2042-650X |
| Popis: | This work aimed to assess the possible beneficial effects of dietary fish oil (FO) on the preexisting adipose tissue dysfunction through the improvement or reversion of the mechanisms underlying oxidative stress and pro-inflammatory cytokines in dyslipemic insulin-resistant rats. Wistar rats were fed a sucrose rich diet (SRD) for 6 months. After that half of the animals continued with the SRD until month 8 while in the other half corn oil was replaced by FO for 2 months (SRD+FO). A reference group consumed a control diet all the time. In epididymal fat pad we analyzed: antioxidant and oxidant enzymes activities; ROS content and glutathione redox state; the protein level of peroxisome proliferator-activated receptor gamma (PPARγ) and the expression and protein levels of uncoupling protein 2 (UCP2) as well as oxidative stress biomarkers and TNF-α and IL-6 plasma levels. Besides, insulin sensitivity and the composition of fatty acid phospholipids of adipose tissue were measured. Compared with the SRD the SRD+FO fed group showed a decrease of fat pad weight and the antioxidant and oxidant enzyme activities and ROS content returned to control values as well as plasma TNF-α and IL-6 levels. FO normalized both the decrease of PPARγ protein and the increase of protein and expression of UCP2. Furthermore, FO increased n-3/n-6 fatty acids ratio in the adipose tissue phospholipids and normalized dyslipidemia and insulin resistance. Finally, these findings reinforce the view that dietary FO may exert a beneficial effect in ameliorating the dyslipidemia and insulin resistance in this animal model. Fil: Selenscig, Dante Alejandro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina Fil: Ferreira Cordoneda, Maria del Rosario. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Chicco, Adriana Graciela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Bolzon, Yolanda Ana Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina |
| Databáze: | OpenAIRE |
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