Comparison of antimicrobial and cytotoxic effects of glutaraldehyde and formocresol
| Autor: | Masayuki Kaga, N. Sue Seale, Charles W. Berry, Sharon D. Hill |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pulpotomy
Formocresols Microbial Sensitivity Tests Pathology and Forensic Medicine Microbiology Tissue culture chemistry.chemical_compound Staphylococcus epidermidis Medicine Candida albicans General Dentistry Dental Pulp Candida Bacteria biology business.industry Streptococcus Fibroblasts Antimicrobial biology.organism_classification Streptococcus mutans Lactobacillus chemistry Glutaral Pulp (tooth) Glutaraldehyde business Neisseria HeLa Cells |
| Zdroj: | Oral Surgery, Oral Medicine, Oral Pathology. 71:89-95 |
| ISSN: | 0030-4220 |
| DOI: | 10.1016/0030-4220(91)90530-p |
| Popis: | The in vitro antimicrobial and cytotoxic concentrations of glutaraldehyde and formocresol were determined. Minimal antimicrobial concentrations of these two agents against selected microbial flora reported in carious primary teeth were 3.125% for glutaraldehyde and 0.75% for formocresol. At a doubling of these concentrations, most organisms, except Candida albicans, Staphylococcus epidermidis, and Streptococcus mutans, were killed by both substances in 30 seconds. Cytotoxicity was evaluated on tissue cultures of pulp fibroblasts and HeLa cells at minimal cidal concentrations and at 10- and 100-fold dilutions. Exposure of pulp fibroblasts and HeLa cells to formocresol and indirect exposure to vapors caused the cells to become atrophic and to form a less dense tissue pattern. Cells directly exposed to glutaraldehyde retained their normal cell shape and tissue pattern, whereas cells indirectly exposed to vapors continued to proliferate. These data showed effective antimicrobial activity at concentrations of 3.125% glutaraldehyde and 0.75% formocresol and suggested that glutaraldehyde may exert a less cytotoxic effect on the immediate and surrounding tissues when used as a pulpotomy agent. |
| Databáze: | OpenAIRE |
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