Direct methods for detection of protein S-nitrosylation
| Autor: | Nelmi O. Devarie-Baez, A. Richard Whorton, Sheng Li, Ming Xian, Dehui Zhang |
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| Rok vydání: | 2013 |
| Předmět: |
S-Nitrosothiols
Proteome Staining and Labeling Chemistry S-Nitrosylation Nitric Oxide Chromatography Affinity Mass Spectrometry Article General Biochemistry Genetics and Molecular Biology Nitric oxide chemistry.chemical_compound Protein S-nitrosylation Biochemistry Biotinylation Direct methods Animals Humans Protein Processing Post-Translational Molecular Biology Cysteine Nitric oxide signaling |
| Zdroj: | Methods. 62:171-176 |
| ISSN: | 1046-2023 |
| DOI: | 10.1016/j.ymeth.2013.04.018 |
| Popis: | S-Nitrosylation of protein cysteine residues is known to be an important mechanism for nitric oxide signaling. However, the detection of protein S-nitrosylation is still challenging due to technical limitations of current methods. This article provides a brief review on recent developments of methods, which directly target S-nitroso moieties for detection. We also describe in detail the protocol of an organophosphine-based biotin labeling of protein S-nitroso moieties. |
| Databáze: | OpenAIRE |
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