Structure-Dependent Interfacial Properties of Chaplin F from Streptomyces coelicolor
| Autor: | Emma L. Prime, Sally L. Gras, Greg G. Qiao, Mina Dokouhaki, Andrew Hung, Li Day |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Circular dichroism Amyloid Hydrophobin lcsh:QR1-502 Peptide Streptomyces coelicolor 02 engineering and technology Biochemistry pressure/area isotherms Article Protein Structure Secondary lcsh:Microbiology 03 medical and health sciences Protein structure Bacterial Proteins Molecular Biology chemistry.chemical_classification atomic force microscopy biology Chemistry self-assembly Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology biology.organism_classification Brewster angle microscopy circular dichroism Random coil Crystallography 030104 developmental biology Isoelectric point 0210 nano-technology Hydrophobic and Hydrophilic Interactions Cysteine |
| Zdroj: | Biomolecules, Vol 7, Iss 3, p 68 (2017) Biomolecules Biomolecules; Volume 7; Issue 3; Pages: 68 |
| Popis: | Chaplin F (Chp F) is a secreted surface-active peptide involved in the aerial growth of Streptomyces. While Chp E demonstrates a pH-responsive surface activity, the relationship between Chp F structure, function and the effect of solution pH is unknown. Chp F peptides were found to self-assemble into amyloid fibrils at acidic pH (3.0 or the isoelectric point (pI) of 4.2), with ~99% of peptides converted into insoluble fibrils. In contrast, Chp F formed short assemblies containing a mixture of random coil and β-sheet structure at a basic pH of 10.0, where only 40% of the peptides converted to fibrils. The cysteine residues in Chp F did not appear to play a role in fibril assembly. The interfacial properties of Chp F at the air/water interface were altered by the structures adopted at different pH, with Chp F molecules forming a higher surface-active film at pH 10.0 with a lower area per molecule compared to Chp F fibrils at pH 3.0. These data show that the pH responsiveness of Chp F surface activity is the reverse of that observed for Chp E, which could prove useful in potential applications where surface activity is desired over a wide range of solution pH. |
| Databáze: | OpenAIRE |
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