A functional test for maternally inherited cadherin in Xenopus shows its importance in cell adhesion at the blastula stage
| Autor: | Janet Heasman, Chris Wylie, Chikako Yoshida-Noro, D. Ginsberg, T. Pratt, K. Goldstone, Benjamin Geiger |
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| Rok vydání: | 1994 |
| Předmět: |
animal structures
Xenopus Gene Expression Biology Xenopus Proteins Xenopus laevis Cell Adhesion Animals RNA Messenger Molecular Biology Genetics Dose-Response Relationship Drug Cadherin Embryogenesis Blastocoel Embryo Blastomere Oligonucleotides Antisense Blastula biology.organism_classification Blotting Northern Cadherins Immunohistochemistry Cell biology Gastrulation Microscopy Electron Blastocyst embryonic structures Microscopy Electron Scanning Female Developmental Biology |
| Zdroj: | Development (Cambridge, England). 120(1) |
| ISSN: | 0950-1991 |
| Popis: | We report here on the consequences of reducing the expression of EP-cadherin at the earliest stages of Xenopus development. Injection of oligodeoxynucleotides antisense to maternal EP-cadherin mRNA into full-grown oocytes reduced the mRNA level in oocytes, and the protein level in blastulae. Adhesion between blastomeres was significantly reduced, as seen in whole embryos, and in assays of the ability of blastomeres to reaggregate in culture. This effect was especially conspicuous in the inner cells of the blastula and included the disruption of the blastocoel. The severity of the EP-cadherin mRNA depletion and of the disaggregation phenotype was dose dependent. This phenotype was rescued by the injection into EP-cadherin mRNA-depleted oocytes of the mRNA coding for a related cadherin, E-cadherin, that is normally expressed at the gastrula stage in the embryonic ectoderm. |
| Databáze: | OpenAIRE |
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