Whole-body kinetic image of a redox probe in mice using Overhauser-enhanced MRI
| Autor: | Tatsuya Naganuma, Kazuhiro Ichikawa, Keiji Yasukawa, Fuminori Hyodo, Ken Ichi Yamada, Noppawan Phumala Morales, Nuttavut Kosem, Hideo Utsumi |
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| Rok vydání: | 2012 |
| Předmět: |
Pyrrolidines
Time Factors Coefficient of variation Kinetics Biochemistry Redox Imaging phantom Cyclic N-Oxides Mice chemistry.chemical_compound Nuclear magnetic resonance Reaction rate constant Pharmacokinetics Physiology (medical) Image Processing Computer-Assisted Animals Phantoms Imaging Chemistry Nitroxyl Magnetic Resonance Imaging Molecular Probes Lipophilicity Female Nitrogen Oxides Spin Labels Oxidation-Reduction |
| Zdroj: | Free Radical Biology and Medicine. 53:328-336 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/j.freeradbiomed.2012.04.026 |
| Popis: | Overhauser-enhanced MRI (OMRI) enables visualization of free radicals in animals based on dynamic nuclear polarization. Real-time data of tissue redox status gathered from kinetic images of redox-sensitive nitroxyl radical probes using OMRI provided both anatomic and physiological information. Phantom experiments demonstrated the linear correlation between the enhancement factor and the concentration of a membrane-impermeable probe, carboxy-PROXYL (3-carboxy-2,2,5,5-tetramethyl- pyrrolidine-1-oxyl). Whole-body OMRI images illustrated the in vivo kinetics of carboxy-PROXYL for 25 min. Initial distribution was observed in lung, heart, liver, and kidney, but not brain, corresponding to its minimal lipophilicity. Based on these images (pixel size, 1.33 × 1.33 mm; slice thickness, 50mm), a time-concentration curve with low coefficient of variance ( |
| Databáze: | OpenAIRE |
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