Mechanism of autonomous synchronization of the circadian KaiABC rhythm
| Autor: | Masaki Sasai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Science Circadian clock Cyanobacteria Models Biological Article Synchronization Computational biophysics 03 medical and health sciences 0302 clinical medicine Rhythm Bacterial Proteins KaiC KaiA Circadian rhythms Circadian rhythm Phosphorylation Mixing (physics) Physics Stochastic Processes Multidisciplinary Circadian Rhythm Signaling Peptides and Proteins Mechanism (biology) Circadian Rhythm 030104 developmental biology Medicine Biological system 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The cyanobacterial circadian clock can be reconstituted by mixing three proteins, KaiA, KaiB, and KaiC, in vitro. In this protein mixture, oscillations of the phosphorylation level of KaiC molecules are synchronized to show the coherent oscillations of the ensemble of many molecules. However, the molecular mechanism of this synchronization has not yet been fully elucidated. In this paper, we explain a theoretical model that considers the multifold feedback relations among the structure and reactions of KaiC. The simulated KaiC hexamers show stochastic switch-like transitions at the level of single molecules, which are synchronized in the ensemble through the sequestration of KaiA into the KaiC–KaiB–KaiA complexes. The proposed mechanism quantitatively reproduces the synchronization that was observed by mixing two solutions oscillating in different phases. The model results suggest that biochemical assays with varying concentrations of KaiA or KaiB can be used to test this hypothesis. |
| Databáze: | OpenAIRE |
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