Human dental pulp pluripotent-like stem cells promote wound healing and muscle regeneration
| Autor: | Domiziana Costamagna, Alessio Rotini, Maurilio Sampaolesi, Raquel Núñez-Toldrà, Aernout Luttun, Maher Atari, Ester Martínez-Sarrà, Sheyla Montori, Carlos Gil-Recio |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Angiogenesis Medicine (miscellaneous) Mice SCID Stem cells Research & Experimental Medicine Extracellular matrix Mice 0302 clinical medicine 10 Technology lcsh:QD415-436 Muscular dystrophy education.field_of_study lcsh:R5-920 NEURAL CREST Anatomy 11 Medical And Health Sciences 3RD MOLAR Cell biology medicine.anatomical_structure DIFFERENTIATION Medicine Research & Experimental 030220 oncology & carcinogenesis Molecular Medicine Heterografts Cytokines SKELETAL-MUSCLE Female Stem cell Growth factors lcsh:Medicine (General) Life Sciences & Biomedicine Adult stem cell Adult Pluripotent Stem Cells Revascularisation Adolescent Population angiogenesis cytokines dental pulp growth factors muscular dystrophy revascularisation stem cells Wound healing medicine (miscellaneous) molecular medicine biochemistry genetics and molecular biology (miscellaneous) cell biology Mice Nude SARCOGLYCAN Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Cell Line lcsh:Biochemistry 03 medical and health sciences medicine Animals Humans education Muscle Skeletal Science & Technology Research Skeletal muscle Cell Biology IN-VITRO Muscular Dystrophy Animal 06 Biological Sciences medicine.disease ENDOTHELIAL-CELLS MUSCULAR-DYSTROPHY Dental pulp 030104 developmental biology Mice Inbred mdx MACROPHAGE PHENOTYPE |
| Zdroj: | Stem Cell Research & Therapy, Vol 8, Iss 1, Pp 1-20 (2017) Stem Cell Research & Therapy |
| ISSN: | 1757-6512 |
| Popis: | Background Dental pulp represents an easily accessible autologous source of adult stem cells. A subset of these cells, named dental pulp pluripotent-like stem cells (DPPSC), shows high plasticity and can undergo multiple population doublings, making DPPSC an appealing tool for tissue repair or maintenance. Methods DPPSC were harvested from the dental pulp of third molars extracted from young patients. Growth factors released by DPPSC were analysed using antibody arrays. Cells were cultured in specific differentiation media and their endothelial, smooth and skeletal muscle differentiation potential was evaluated. The therapeutic potential of DPPSC was tested in a wound healing mouse model and in two genetic mouse models of muscular dystrophy (Scid/mdx and Sgcb-null Rag2-null γc-null). Results DPPSC secreted several growth factors involved in angiogenesis and extracellular matrix deposition and improved vascularisation in all three murine models. Moreover, DPPSC stimulated re-epithelialisation and ameliorated collagen deposition and organisation in healing wounds. In dystrophic mice, DPPSC engrafted in the skeletal muscle of both dystrophic murine models and showed integration in muscular fibres and vessels. In addition, DPPSC treatment resulted in reduced fibrosis and collagen content, larger cross-sectional area of type II fast-glycolytic fibres and infiltration of higher numbers of proangiogenic CD206+ macrophages. Conclusions Overall, DPPSC represent a potential source of stem cells to enhance the wound healing process and slow down dystrophic muscle degeneration. Electronic supplementary material The online version of this article (doi:10.1186/s13287-017-0621-3) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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