Interspecies cell fusion between mouse embryonic stem cell and porcine pluripotent cell
| Autor: | Yan Li, Zhonghua Liu, Gerelchimeg Bou, Renyue Wei, Jia Guo, Fang Yuan, Xuechun Li |
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| Rok vydání: | 2021 |
| Předmět: |
Pluripotent Stem Cells
Somatic cell Swine Cell Germ layer Biology Cell Line Cell Fusion 03 medical and health sciences Mice 0302 clinical medicine Endocrinology medicine Animals Induced pluripotent stem cell Chromosome Aberrations 030219 obstetrics & reproductive medicine Cell fusion 0402 animal and dairy science Cell Differentiation Mouse Embryonic Stem Cells 04 agricultural and veterinary sciences Cellular Reprogramming 040201 dairy & animal science Embryonic stem cell Cell biology medicine.anatomical_structure Animal Science and Zoology Stem cell Reprogramming Biotechnology |
| Zdroj: | Reproduction in domestic animals = ZuchthygieneREFERENCES. 56(8) |
| ISSN: | 1439-0531 |
| Popis: | In the area of stem cell research, fusion of somatic cells into pluripotent cells such as mouse embryonic stem (ES) cells induces reprogramming of the somatic nucleus and can be used to study the effect of trans-acting factors from the pluripotent cell on the pluripotent state of somatic nucleus. As many other groups, we previously established a porcine pluripotent cell line at a low potential. Therefore, here, we performed experiments to investigate if the fusion with mouse ES cell could improve the pluripotent state of porcine pluripotent cell. Our data showed that resultant mouse-porcine interspecies fused cells are AP positive, and could be passaged up to 20 passages. Different degrees of increases in expression of porcine pluripotent genes proved that pig-origin gene network can be programmed by mouse ES. Further differentiation study also confirmed these fused cells' potential to form three germ layers. However, unexpectedly, we found that chromosome loss and aberrant (especially in porcine chromosomes) is severe after the cell fusion, implying that interspecies cell fusion may be not suitable to study porcine pluripotency without additional supportive conditions for genome stabilization. |
| Databáze: | OpenAIRE |
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