Preimplantation genetic diagnosis for the treatment of failed in vitro fertilization–embryo transfer and habitual abortion
| Autor: | Fulvio Zullo, Brian Dale, Fulvio Cappiello, Martin Wilding, Robert Forman, George Hogewind, Loredana Di Matteo |
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| Přispěvatelé: | Wilding, M., Forman, R., Hogewind, G., DI MATTEO, Loredana, Zullo, F., Cappiello, F., Dale, B., Wilding, Martin, Forman, Robert, Hogewind, George, Di Matteo, Loredana, Zullo, Fulvio, Cappiello, Fulvio, Dale, Brian |
| Rok vydání: | 2004 |
| Předmět: |
Adult
Abortion Habitual medicine.medical_specialty spontaneous pregnancy lo medicine.medical_treatment spontaneous pregnancy loss Fertilization in Vitro Controlled ovarian hyperstimulation Biology Abortion Preimplantation genetic diagnosis Pregnancy medicine Humans Prospective Studies Prospective cohort study fluorescence in situ hybridization Preimplantation Diagnosis reproductive and urinary physiology Preimplantation Diagnosi Gynecology In vitro fertilisation Obstetrics and Gynecology Embryo Embryo Transfer medicine.disease human embryo Embryo transfer Prospective Studie Reproductive Medicine embryonic structures Female in vitro fertilization Human |
| Zdroj: | Fertility and Sterility. 81:1302-1307 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/j.fertnstert.2003.10.028 |
| Popis: | Objective To apply preimplantation genetic diagnosis (PGD) for the treatment of patients with a history of failed IVF-ET or habitual aborters. Design Prospective clinical study. Setting Tertiary center for assisted reproduction. Patient(s) Ninety-four couples with failed IVF-ET after >2 IVF cycles and 64 couples with >2 spontaneous abortions. Intervention(s) Patients were prepared for oocyte retrieval using standard controlled ovarian hyperstimulation protocols after standard laboratory techniques. Blastomeres from 6- to 8-cell embryos were analysed using fluorescence in situ hybridization with commercial chromosomal probes, and normoploid embryos were transferred on day 3 after fertilization. Main outcome measure(s) Pregnancy and implantation rates and live births. Result(s) Both 3- and 5-probe PGD resulted in a significantly higher outcome than controls for failed IVF-ET. Five-probe PGD appeared to be more suitable for habitual aborters. Conclusion(s) This pilot study suggests that 3-probe PGD is a valid option for failed IVF-ET patients. The use of five or more probes is indicated for habitual aborters. |
| Databáze: | OpenAIRE |
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