Urinary Estrone Conjugate and Pregnanediol 3-Glucuronide Enzyme Immunoassays for Population Research
| Autor: | Eleanor Brindle, C. J. Munro, Fortune Kohen, Nancy A. Klein, Michael R. Soules, James W. Wood, Jane B. Shofer, Kenneth L. Campbell, Bill L. Lasley, Darryl J. Holman, Kathleen A. O'Connor |
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| Rok vydání: | 2003 |
| Předmět: |
Adult
medicine.medical_specialty Estrone medicine.drug_class Metabolite Fluoroimmunoassay Clinical Biochemistry Urine Luteal phase Biology Specimen Handling Immunoenzyme Techniques chemistry.chemical_compound Internal medicine Follicular phase medicine Humans Mass Screening Bangladesh Estrogens Conjugated (USP) Estradiol Estriol Biochemistry (medical) Middle Aged United States Estrone Conjugate Endocrinology chemistry Estrogen Pregnanediol Female |
| Zdroj: | Clinical Chemistry. 49:1139-1148 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1373/49.7.1139 |
| Popis: | Background: Monitoring of reproductive steroid hormones at the population level requires frequent measurements, hormones or metabolites that remain stable under less than ideal collection and storage conditions, a long-term supply of antibodies, and assays useful for a range of populations. We developed enzyme immunoassays for urinary pregnanediol 3-glucuronide (PDG) and estrone conjugates (E1Cs) that meet these criteria. Methods: Enzyme immunoassays based on monoclonal antibodies were evaluated for specificity, detection limit, parallelism, recovery, and imprecision. Paired urine and serum specimens were analyzed throughout menstrual cycles of 30 US women. Assay application in different populations was examined with 23 US and 42 Bangladeshi specimens. Metabolite stability in urine was evaluated for 0–8 days at room temperature and for 0–10 freeze-thaw cycles. Results: Recoveries were 108% for the PDG assay and 105% for the E1C assay. Serially diluted specimens exhibited parallelism with calibration curves in both assays. Inter- and intraassay CVs were Conclusions: These enzyme immunoassays can be used for the field conditions and population variation in hormone metabolite concentrations encountered in cross-cultural research. |
| Databáze: | OpenAIRE |
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