Chronic-pain-associated astrocytic reaction in the spinal cord dorsal horn of human immunodeficiency virus-infected patients
| Autor: | Benjamin B. Gelman, Yuqiang Shi, Joshua G. Lisinicchia, Shao Jun Tang |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty MAP Kinase Signaling System Interleukin-1beta Cell Count HIV Infections Nerve Tissue Proteins macromolecular substances S100 Calcium Binding Protein beta Subunit Biology Article Proinflammatory cytokine Spinal Cord Dorsal Horn Glial Fibrillary Acidic Protein medicine Humans Nerve Growth Factors Analysis of Variance CD11b Antigen Microglia Glial fibrillary acidic protein Tumor Necrosis Factor-alpha General Neuroscience S100 Proteins Chronic pain Middle Aged medicine.disease Spinal cord medicine.anatomical_structure Nerve growth factor Spinal Cord Astrocytes Postmortem Changes Immunology biology.protein Tumor necrosis factor alpha Chronic Pain |
| Zdroj: | The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(32) |
| ISSN: | 1529-2401 |
| Popis: | Studies with animal models have suggested that reaction of glia, including microglia and astrocytes, critically contributes to the development and maintenance of chronic pain. However, the involvement of glial reaction in human chronic pain is unclear. We performed analyses to compare the glial reaction profiles in the spinal dorsal horn (SDH) from three cohorts of sex- and age-matched human postmortem tissues: (1) HIV-negative patients, (2) HIV-positive patients without chronic pain, and (3) HIV patients with chronic pain. Our results indicate that the expression levels of CD11b and Iba1, commonly used for labeling microglial cells, did not differ in the three patient groups. However, GFAP and S100β, often used for labeling astrocytes, were specifically upregulated in the SDH of the “pain-positive” HIV patients but not in the “pain-negative” HIV patients. In addition, proinflammatory cytokines, TNFα and IL-1β, were specifically increased in the SDH of pain-positive HIV patients. Furthermore, proteins in the MAPK signaling pathway, including pERK, pCREB and c-Fos, were also upregulated in the SDH of pain-positive HIV patients. Our findings suggest that reaction of astrocytes in the SDH may play a role during the maintenance phase of HIV-associated chronic pain. |
| Databáze: | OpenAIRE |
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