Molecular cloning and characterization of NcROP2Fam-1, a member of the ROP2 family of rhoptry proteins in Neospora caninum that is targeted by antibodies neutralizing host cell invasion in vitro
| Autor: | Karim Debache, Andrew Hemphill, Ferial Alaeddine, Christophe Guionaud |
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| Rok vydání: | 2013 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Protozoan Proteins Antibodies Protozoan Antigens Protozoan Molecular cloning 03 medical and health sciences Antigen Chlorocebus aethiops Extracellular Animals Humans Amino Acid Sequence Cloning Molecular Vero Cells Host cell nucleus 030304 developmental biology 0303 health sciences Rhoptry biology 630 Agriculture Base Sequence 030306 microbiology Intracellular parasite Neospora Membrane Proteins Sequence Analysis DNA DNA Protozoan Fibroblasts biology.organism_classification Molecular biology Neospora caninum 3. Good health Transport protein Protein Structure Tertiary Protein Transport Infectious Diseases Vacuoles Animal Science and Zoology Parasitology RNA Protozoan |
| Zdroj: | Parasitology Alaeddine, Ferial; Hemphill, Andrew; Debache, Karim; Guionaud, Christophe (2013). Molecular cloning and characterization of NcROP2Fam-1, a member of the ROP2 family of rhoptry proteins in Neospora caninum that is targeted by antibodies neutralizing host cell invasion in vitro. Parasitology, 140(8), pp. 1033-1050. Cambridge University Press 10.1017/S0031182013000383 |
| DOI: | 10.1017/S0031182013000383 |
| Popis: | SUMMARYRecent publications demonstrated that a fragment of aNeospora caninumROP2 family member antigen represents a promising vaccine candidate. We here report on the cloning of the cDNA encoding this protein,N. caninumROP2 family member 1 (NcROP2Fam-1), its molecular characterization and localization. The protein possesses the hallmarks of ROP2 family members and is apparently devoid of catalytic activity. NcROP2Fam-1 is synthesized as a pre-pro-protein that is matured to 2 proteins of 49 and 55 kDa that localize to rhoptry bulbs. Upon invasion the protein is associated with the nascent parasitophorous vacuole membrane (PVM), evacuoles surrounding the host cell nucleus and, in some instances, the surface of intracellular parasites. Staining was also observed within the cyst wall of ‘cysts’ producedin vitro. Interestingly, NcROP2Fam-1 was also detected on the surface of extracellular parasites entering the host cells and antibodies directed against NcROP2Fam-1-specific peptides partially neutralized invasionin vitro. We conclude that, in spite of the general belief that ROP2 family proteins are intracellular antigens, NcROP2Fam-1 can also be considered as an extracellular antigen, a property that should be taken into account in further experiments employing ROP2 family proteins as vaccines. |
| Databáze: | OpenAIRE |
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