Augmented mobilization and collection of CD34+ hematopoietic cells from normal human volunteers stimulated with granulocyte-colony-stimulating factor by single-dose administration of AMD3100, a CXCR4 antagonist

Autor: Jeffrey Christensen, David C. Dale, Christine Dehner, Brent L. Wood, Elin Rodger, Gary Calandra, Karin Badel, Thomas H. Price, Hal E. Broxmeyer, W. Conrad Liles
Rok vydání: 2005
Předmět:
Zdroj: Transfusion. 45(3)
ISSN: 0041-1132
Popis: BACKGROUND: AMD3100, a selective antagonist of CXCR4, rapidly mobilizes CD34+ hematopoietic progenitor cells (HPCs) from marrow to peripheral blood with minimal side effects. STUDY DESIGN AND METHODS: To further investigate potential clinical utility of AMD3100 for CD34+ cell mobilization and collection, a Phase I study in normal volunteers was performed examining single-dose administration of AMD3100 alone and in combination with a standard 5-day granulocyte–colony-stimulating factor (G–CSF) regimen. RESULTS: AMD3100 (160 µg/kg × 1 on Day 5) significantly increased both G–CSF-stimulated (10 µg/kg/day) mobilization of CD34+ cells (3.8-fold) and leukapheresis yield of CD34+ cells. Moreover, collection of CD34+ cells was comparable between individuals mobilized by a single-dose regimen of AMD3100 (240 µg/kg) and individuals mobilized with a 5-day regimen of G–CSF. AMD3100-mobilized leukapheresis products contained significantly greater numbers of T and B cells compared to G–CSF-stimulated leukapheresis products. CONCLUSION: These findings indicate that AMD3100 can be used alone or as an adjunct to G–CSF to mobilize cells for HPC transplantation.
Databáze: OpenAIRE
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