Mutations of hepatitis C virus 1b NS5A 2209-2248 amino acid sequence is not a predictive factor for response to interferon-alpha therapy and development of hepatocellular carcinoma
| Autor: | Si Hyun Bae, Sang Bok Cha, Chang Don Lee, Doo Ho Park, Boo Sung Kim, Duck Gi Yoo, Jong Young Choi, Young Min Park, Jin Mo Yang, Byung Hun Byun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Carcinoma Hepatocellular Genotype Hepacivirus Hepatitis C virus Molecular Sequence Data Alpha interferon medicine.disease_cause Antiviral Agents Interferon medicine Humans Amino Acid Sequence NS5A Codon Aged biology Base Sequence Reverse Transcriptase Polymerase Chain Reaction Liver Neoplasms Interferon-alpha General Medicine Hepatitis C Hepatitis C Chronic Middle Aged medicine.disease biology.organism_classification Prognosis Virology digestive system diseases Hepatocellular carcinoma Mutation Female medicine.drug Research Article |
| Zdroj: | Journal of Korean Medical Science |
| ISSN: | 1598-6357 1011-8934 |
| Popis: | Genetic changes between codons 2209 and 2248 of NS5A of genotype 1b hepatitis C virus (HCV-1b) have been reported to be associated with the sensitivity to interferon-alpha (IFN-alpha). The present study was performed to analyze such relationship in Korean patients with chronic hepatitis C and HCV-1b (n=19), including 12 chronic hepatitis C patients treated with IFN-alpha, 3 chronic hepatitis C patients without treatment as controls, and 4 patients with hepatocellular carcinoma (HCC). Two serum samples, before and after the treatment, were analyzed for the mutations by reverse transcription-polymerase chain reaction, cloning and sequencing. The mutations were identified in 32% (6/19), including five intermediate type (1-3 mutations) and one mutant type (4 or more). In 12 patients treated with IFN-alpha, the number of amino acid substitutions in NS5A2209-2248 was not associated with outcome of the treatment. Two HCV isolates with NS5A2209-2248 mutations from HCC patients were intermediate type. These results do not support that the NS5A2209-2248 determines interferon sensitivity of HCV-1b and that the mutations is associated with development of HCC. |
| Databáze: | OpenAIRE |
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