Subtelomeric factors antagonize telomere anchoring and Tel1-independent telomere length regulation

Autor: Eric Gilson, Griet Van Houwe, Florence Hediger, Susan M. Gasser, Anne-Sophie Berthiau
Přispěvatelé: Université de Genève = University of Geneva (UNIGE), Unité mixte de recherche biologie moléculaire de la cellule, École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Moléculaire de la Cellule (LBMC), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Cachan (ENS Cachan), Friedrich Miescher Institute for Biomedical Research, Université de Genève (UNIGE), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), ProdInra, Migration
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Telomerase
Saccharomyces cerevisiae Proteins
Nuclear Envelope
Saccharomyces cerevisiae
TELOMERE
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Protein Serine-Threonine Kinases
DNA-binding protein
General Biochemistry
Genetics and Molecular Biology
Article
Fungal Proteins
03 medical and health sciences
Transactivation
0302 clinical medicine
TELOMERASE
Dinucleotide Repeats
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
Molecular Biology
Silent Information Regulator Proteins
Saccharomyces cerevisiae
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Genetics
Telomere-binding protein
0303 health sciences
Fungal protein
General Immunology and Microbiology
biology
General Neuroscience
Intracellular Signaling Peptides and Proteins
Herpes Simplex Virus Protein Vmw65
Subtelomere
biology.organism_classification
GENOMIQUE
Telomere
DNA-Binding Proteins
NUCLEAR ORGANIZATION
SIR4
Chromosomes
Fungal
030217 neurology & neurosurgery
yKu
Zdroj: EMBO Journal
EMBO Journal, 2006, 25 (4), pp.857-867
EMBO Journal, EMBO Press, 2006, 25 (4), pp.857-867
ISSN: 0261-4189
1460-2075
Popis: Yeast telomeres are anchored at the nuclear envelope (NE) through redundant pathways that require the telomere-binding factors yKu and Sir4. Significant variation is observed in the efficiency with which different telomeres are anchored, however, suggesting that other forces influence this interaction. Here, we show that subtelomeric elements and the insulator factors that bind them antagonize the association of telomeres with the NE. This is detectable when the redundancy in anchoring pathways is compromised. Remarkably, these same conditions lead to a reduction in steady-state telomere length in the absence of the ATM-kinase homologue Tel1. Both the delocalization of telomeres and reduction in telomere length can be induced by targeting of Tbf1 or Reb1, or the viral transactivator VP16, to a site 23 kb away from the TG repeat. This correlation suggests that telomere anchoring and a Tel1-independent pathway of telomere length regulation are linked, lending a functional significance to the association of yeast telomeres with the NE.
Databáze: OpenAIRE
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