Physioxic human cell culture improves viability, metabolism, and mitochondrial morphology while reducing DNA damage
| Autor: | Brianna D. Guild, James Uniacke, Sara Timpano, Philip J. Medeiros, Gaelan Melanson, Shannon L. J. Sproul, Erin J. Specker |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Antioxidant Cell Survival DNA damage medicine.medical_treatment chemistry.chemical_element medicine.disease_cause Biochemistry Oxygen Antioxidants 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Genetics medicine Humans Molecular Biology Cells Cultured Cell Proliferation Chemistry Metabolism Mitochondria Cell biology Oxidative Stress 030104 developmental biology mitochondrial fusion Cell culture Toxicity Oxidation-Reduction 030217 neurology & neurosurgery Oxidative stress DNA Damage Biotechnology |
| Zdroj: | The FASEB Journal. 33:5716-5728 |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fj.201802279r |
| Popis: | Multicellular organisms balance oxygen delivery and toxicity by having oxygen pass through several barriers before cellular delivery. In human cell culture, these physiologic barriers are removed, exposing cells to higher oxygen levels. Human cells cultured in ambient air may appear normal, but this is difficult to assess without a comparison at physiologic oxygen. Here, we examined the effects of culturing human cells throughout the spectrum of oxygen availability on oxidative damage to macromolecules, viability, proliferation, the antioxidant and DNA damage responses, metabolism, and mitochondrial fusion and morphology. We surveyed 4 human cell lines cultured for 3 d at 7 oxygen conditions between 1 and 21% O2. We show that oxygen levels and cellular benefit are not inversely proportional, but the benefit peaks within the physioxic range. Normoxic cells are in a perpetual state of responding to damaged macromolecules and mitochondrial networks relative to physioxic cells, which could compromise an investigation. These data contribute to the concept of an optimal oxygen availability for cell culture in the physioxic range where the oxygen is not too high to reduce oxidative damage, and not too low for efficient oxidative metabolism, but just right: the Goldiloxygen zone.-Timpano, S., Guild, B. D., Specker, E. J., Melanson, G., Medeiros, P. J., Sproul, S. L. J., Uniacke, J. Physioxic human cell culture improves viability, metabolism, and mitochondrial morphology while reducing DNA damage. |
| Databáze: | OpenAIRE |
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