Analysis of specific IgM responses in secondary dengue virus infections: levels and positive rates in comparison with primary infections
| Autor: | Sumalee Chanama, Areerat Sa-gnasang, Surapee Anantapreecha, Ichiro Kurane, Pathom Sawanpanyalert, Atchareeya A-nuegoonpipat |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Adult
Time Factors Adolescent Secondary infection Enzyme-Linked Immunosorbent Assay Dengue virus Antibodies Viral medicine.disease_cause Virus Serology Dengue fever Dengue Flaviviridae Virology medicine Humans Child biology Reverse Transcriptase Polymerase Chain Reaction Infant Dengue Virus biology.organism_classification medicine.disease Flavivirus Infectious Diseases Immunoglobulin M Child Preschool Acute Disease Immunology Viral disease |
| Zdroj: | Journal of Clinical Virology. 31:185-189 |
| ISSN: | 1386-6532 |
| DOI: | 10.1016/j.jcv.2004.03.005 |
| Popis: | Background: Dengue viruses are a serious cause of illness in tropical and subtropical areas of the world. Laboratory diagnosis is essential for confirmation of dengue virus infections. Detection of specific IgM by IgM-capture enzymed-linked immunoassay (ELISA) has been widely used as a main serological diagnostic technique. Objectives: The levels of specific IgM in secondary dengue virus infections were compared with those in primary infections. Study design: A total of 1780 samples collected from 924 confirmed dengue cases were tested for anti-dengue IgM by IgM-capture ELISA. Results and conclusions: Specific IgM was detected in all the cases with primary dengue virus infection on disease day 9 or later. However, specific IgM cannot be detected in 28% (204/716) of the cases in secondary infections. The average titers of IgM were higher in primary infections than in secondary infections. The results confirmed that IgM detection is a reliable serological diagnostic test in primary dengue virus infections. Although IgM detection is also a useful test, other serological diagnostic tests or tests for dengue virus detection are necessary for confirmation of all the secondary dengue virus infections. |
| Databáze: | OpenAIRE |
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