Double-tracer autoradiographic study of protein synthesis and glucose consumption in rats with focal cerebral ischemia
| Autor: | Nils Henrik Diemer, Torben Balchen, Torben Bruhn, Thomas Christensen |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
Radioisotope Dilution Technique Ischemia Blood Pressure Nerve Tissue Proteins Deoxyglucose Tritium chemistry.chemical_compound Leucine Reference Values Quinoxalines TRACER medicine.artery Occlusion Animals Medicine Carbon Radioisotopes Rats Wistar Trichloroacetic acid Quantitative Autoradiography business.industry Penumbra Brain General Medicine Metabolism medicine.disease Rats Glucose Neurology chemistry Ischemic Attack Transient Anesthesia Middle cerebral artery Autoradiography Neurology (clinical) Dizocilpine Maleate business Nuclear medicine Excitatory Amino Acid Antagonists |
| Zdroj: | University of Copenhagen |
| ISSN: | 1743-1328 0161-6412 |
| Popis: | A double-tracer autoradiographic method for simultaneous measurement of regional glucose utilization (rCMRglc) and regional protein synthesis (PS) in consecutive brain sections is described and applied to study the metabolism of the ischemic penumbra 2 h after occlusion of the middle cerebral artery (MCAO) in rats. In halothane anesthesia, the left middle cerebral artery was permanently occluded. Two hours after MCAO an i.v. bolus injection of 14C-deoxyglucose and 3H-leucine was given and circulated for 45 min. Two sets of brain sections were processed for quantitative autoradiography. Neighboring brain sections exposed an X-ray film (3H-insensitive), and a 3H-sensitive for determination of rCMRglc and PS, respectively. Sections for PS determination were washed in trichloroacetic acid (TCA) prior to film exposure in order to remove 14C-deoxyglucose and unincorporated 3H-leucine. Regional rates of PS and glucose utilization were measured by densitometric image analysis. Normal rates of metabolism were defined as mean +/- 2 SD of values in the non-ischemic cortex. The volumes of ischemic cortex displaying normal rates of PS and glucose utilization, respectively, were measured. The cortical volume with normal PS was significantly less than that of normal rCMRglc: 142 (127-147) mm3 vs. 203 (184-206) mm3. Treatment with the glutamate antagonists MK-801 (1 mg kg-1) and NBQX (30 mg kg-1 x 2) did not significantly change this, although MK-801 tended to reduce the size of the metabolic penumbra calculated as the difference between ischemic cortex with reduced PS and ischemic cortex with reduced rCMRglc. |
| Databáze: | OpenAIRE |
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