Genome wide identification of C1-2i zinc finger proteins and their response to abiotic stress in hexaploid wheat
| Autor: | Arnaud Cheuk, Mario Houde |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Light Quantitative trait locus 01 natural sciences Genome 03 medical and health sciences Gene Expression Regulation Plant Stress Physiological Genetics Aegilops tauschii Molecular Biology Gene Triticum Plant Proteins 2. Zero hunger Abiotic component Zinc finger biology Abiotic stress food and beverages Chromosome Mapping Zinc Fingers General Medicine Hydrogen Peroxide biology.organism_classification Droughts 030104 developmental biology Triticum urartu Transcriptome Genome Plant 010606 plant biology & botany |
| Zdroj: | Molecular genetics and genomics : MGG. 291(2) |
| ISSN: | 1617-4623 |
| Popis: | The C1-2i wheat Q-type C2H2 zinc finger protein (ZFP) transcription factor subclass has been reported to play important roles in plant stress responses. This subclass of ZFPs has not been studied in hexaploid wheat (Triticum aestivum) and we aimed to identify all members of this subclass and evaluate their responses to different abiotic stresses causing oxidative stress. Exploiting the recently published wheat draft genome sequence, we identified 53 members (including homoeologs from A, B and D genomes) of the C1-2i wheat Q-type C2H2 ZFPs (TaZFPs) representing 21 genes. Evolution analysis revealed that 9 TaZFPs members are directly inherited from the parents Triticum urartu and Aegilops tauschii, while 15 diverged through neoploidization events. This TaZFP subclass is responsive to the oxidative stress generator H2O2 and to high light, drought stress and flooding. Most TaZFPs are responsive to H2O2 (37/53), high light (44/53), flooding (31/53) or drought (37/53); 32 TaZFPs were up-regulated by at least 3 stresses and 16 were responsive to all stresses tested. A large number of these TaZFPs were physically mapped on different wheat draft genome sequences with known markers useful for QTL mapping. Our results show that the C1-2i subclass of TaZFPs is associated with responses to different abiotic stresses and that most TaZFPs (30/53 or 57 %) are located on group 5 chromosomes known to be involved in environment adaptation. Detailed characterization of these novel wheat TaZFPs and their association to QTL or eQTL may help to design wheat cultivars with improved tolerance to abiotic stress. |
| Databáze: | OpenAIRE |
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