Derivation of Human Midbrain-Specific Organoids from Neuroepithelial Stem Cells
| Autor: | Ronan M. T. Fleming, Ibrahim Boussaad, Anna S. Monzel, Jens Christian Schwamborn, Javier Jarazo, Siham Hachi, Edinson Lucumi Moreno, Jonas Walter, Kathrin Hemmer, Emanuel Berger, Silvia Bolognin, Inga Brüggemann, Thea van Wuellen, Lisa M. Smits |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Neurogenesis organoid Neuroepithelial Cells midbrain Biology Biochemistry Midbrain 03 medical and health sciences Mesencephalon stem cells Report Genetics Organoid medicine Humans lcsh:QH301-705.5 Cells Cultured Myelin Sheath neural stem cells lcsh:R5-920 Dopaminergic Neurons Oligodendrocyte differentiation Cell Biology Human brain Anatomy Neural stem cell Neuroepithelial cell Organoids 030104 developmental biology medicine.anatomical_structure nervous system lcsh:Biology (General) Parkinson’s disease Stem cell lcsh:Medicine (General) Neuroscience Developmental Biology Cerebral organoid |
| Zdroj: | Stem Cell Reports, Vol 8, Iss 5, Pp 1144-1154 (2017) Stem Cell Reports, 8(5), 1144-1154. Cell Press Stem Cell Reports |
| ISSN: | 2213-6711 |
| Popis: | Summary Research on human brain development and neurological diseases is limited by the lack of advanced experimental in vitro models that truly recapitulate the complexity of the human brain. Here, we describe a robust human brain organoid system that is highly specific to the midbrain derived from regionally patterned neuroepithelial stem cells. These human midbrain organoids contain spatially organized groups of dopaminergic neurons, which make them an attractive model for the study of Parkinson’s disease. Midbrain organoids are characterized in detail for neuronal, astroglial, and oligodendrocyte differentiation. Furthermore, we show the presence of synaptic connections and electrophysiological activity. The complexity of this model is further highlighted by the myelination of neurites. The present midbrain organoid system has the potential to be used for advanced in vitro disease modeling and therapy development. Graphical Abstract Highlights • Generation of a human in vitro midbrain model from neural precursor cells • Midbrain organoids show neuronal, astroglial, and oligodendrocyte differentiation • Midbrain organoids contain spatially organized groups of dopaminergic neurons • Detection of synaptic connections, electrophysiological activity, and myelination Monzel and colleagues demonstrate the derivation of human midbrain-specific brain organoids from lineage-restricted human neural precursor cells. They further show that the stem cells can self-organize into spatially patterned groups of dopaminergic neurons. Moreover, the presence of synaptic connections, myelinated of neurites, and electrophysiological activity of neurons is demonstrated. |
| Databáze: | OpenAIRE |
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