Functional Attributes of Myco-Synthesized Silver Nanoparticles from Endophytic Fungi: A New Implication in Biomedical Applications
| Autor: | Sathishkumar Gnanasekar, Prabu Kumar Seetharaman, Kamil Kuca, Rajiv Periakaruppan, Rajkuberan Chandrasekaran, Sivaramakrishnan Sivaperumal, Kamel A. Abd-Elsalam, Martin Vališ |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
endophytic fungi silver nanoparticles General Immunology and Microbiology Absorption spectroscopy QH301-705.5 Nanoparticle 02 engineering and technology Biology 021001 nanoscience & nanotechnology General Biochemistry Genetics and Molecular Biology Silver nanoparticle Article Nanomaterials Absorbance 03 medical and health sciences 030104 developmental biology antibacterial activity Transmission electron microscopy Biology (General) Surface plasmon resonance 0210 nano-technology General Agricultural and Biological Sciences Antibacterial activity Nuclear chemistry |
| Zdroj: | Biology Volume 10 Issue 6 Biology, Vol 10, Iss 473, p 473 (2021) |
| ISSN: | 2079-7737 |
| DOI: | 10.3390/biology10060473 |
| Popis: | Simple Summary Greener fabrication of silver nanoparticles has received extensive attention owing to their low cytotoxicity, tunable surface features, and stability. Fabrication of AgNPs using natural entities has received substantial consideration as an alternative to toxic chemicals. In this study, we have screened the potential of an endophytic fungi Penicillium oxalicum strain LA-1 for the synthesis of AgNPs and analyzed their potential multipurpose values in biology and medicine. Abstract To develop a benign nanomaterial from biogenic sources, we have attempted to formulate and fabricate silver nanoparticles synthesized from the culture filtrate of an endophytic fungus Penicillium oxalicum strain LA-1 (PoAgNPs). The synthesized PoAgNPs were exclusively characterized through UV–vis absorption spectroscopy, Fourier Transform Infra-Red spectroscopy (FT-IR), X-ray powder diffraction (XRD), and Transmission Electron Microscopy (TEM) with energy dispersive X-ray spectroscopy (EDX). The synthesized nanoparticles showed strong absorbance around 430 nm with surface plasmon resonance (SPR) and exhibited a face-centered cubic crystalline nature in XRD analysis. Proteins presented in the culture filtrate acted as reducing, capping, and stabilization agents to form PoAgNPs. TEM analysis revealed the generation of polydispersed spherical PoAgNPs with an average size of 52.26 nm. The PoAgNPs showed excellent antibacterial activity against bacterial pathogens. The PoAgNPs induced a dose-dependent cytotoxic activity against human adenocarcinoma breast cancer cell lines (MDA-MB-231), and apoptotic morphological changes were observed by dual staining. Additionally, PoAgNPs demonstrated better larvicidal activity against the larvae of Culex quinquefasciatus. Moreover, the hemolytic test indicated that the as-synthesized PoAgNPs are a safe and biocompatible nanomaterial with versatile bio-applications. |
| Databáze: | OpenAIRE |
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