Development and Evaluation of Epitope-Blocking ELISA for Detection of Antibodies against Contagious Caprine Pleuropneumonia in Goat Sera
Autor: | Yao Mathurin Koffi, Takele A. Tefera, Ethel Chitsungo, Nduta Mwangi, Baziki Jean de Dieu, Naomi Maina, Rume Veronica Nwankpa, Cisse Rahamatou Moustapha Boukary, Bodjo S. Charles, Nick Nwankpa |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
040301 veterinary sciences
medicine.drug_class cut-off value blocking-elisa Monoclonal antibody Article Epitope Mycoplasma capricolum 0403 veterinary science 03 medical and health sciences Contagious caprine pleuropneumonia medicine Small ruminant 030304 developmental biology 0303 health sciences lcsh:Veterinary medicine General Veterinary biology Cut off value 04 agricultural and veterinary sciences biology.organism_classification medicine.disease Virology Vaccination Campaigns monoclonal antibody sensitivity and specificity biology.protein lcsh:SF600-1100 blocking-ELISA Antibody |
Zdroj: | Veterinary Sciences, Vol 6, Iss 4, p 82 (2019) Veterinary Sciences; Volume 6; Issue 4; Pages: 82 Veterinary Sciences |
ISSN: | 2306-7381 |
Popis: | Enzyme linked immunosorbent assays (ELISAs) have been developed for the detection of antibodies against contagious caprine pleuropneumonia (CCPP), the causative agent of which is Mycoplasma capricolum subsp. Capripneumoniae (Mccp). The currently available commercial CCPP competitive ELISA (CCPP cELISA) kit produced and supplied by IDEXX Company (Westbrook, Maine, United States) is relatively expensive for most African laboratories. To address this issue and provide a variety of choices, a sensitive and specific blocking-ELISA (b-ELISA) test to detect antibodies against CCPP was developed. We describe the newly developed CCPP blocking-ELISA based on the blocking of an epitope of a monoclonal antibody (Mccp-25) by a positive serum sample against the Mccp protein coated on a plate. The Percentage Inhibition (PI) cut-off value for the CCPP b-ELISA was set at 50 using 466 CCPP negative and 84 CCPP positive small ruminant sera. Of the negative sera, 307 were obtained from the Botswana National Veterinary Laboratory (BNVL) and 159 from the Friedrich-Loeffler-Institute (FLI) Germany. The 84 positive sera samples came from experimentally vaccinated goats at the AU-PANVAC facility in Debre-Zeit, Ethiopia. The relative diagnostic sensitivity and specificity of the CCPP b-ELISA was 93% and 88%, respectively. This test result indicated good correlation with that of the commercial CCPP cELISA by IDEXX Company (Westbrook, Maine, United States) with a Cohen’s κ agreement of κ agreement of 0.85. The newly developed CCPP b-ELISA will be useful in the detection of antibodies for the diagnosis CCPP and for sero-surveillance during vaccination campaigns. |
Databáze: | OpenAIRE |
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