Cell type-specific effects of p27KIP1 loss on retinal development
| Autor: | Fumi Sato, Mariko Ogawa, Fuminori Saitoh, Hiroki Fujieda, Norihiro Sudou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell type Cyclin-dependent kinase inhibitor p27KIP1 Cellular differentiation Ectopic cell cycle reentry lcsh:RC346-429 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience Cyclin-dependent kinase medicine Progenitor cell lcsh:Neurology. Diseases of the nervous system Retina Cone death biology Cell growth Cell cycle Cell biology 030104 developmental biology medicine.anatomical_structure biology.protein Retinal development sense organs Muller glia 030217 neurology & neurosurgery |
| Zdroj: | Neural Development, Vol 12, Iss 1, Pp 1-12 (2017) |
| ISSN: | 1749-8104 |
| DOI: | 10.1186/s13064-017-0094-1 |
| Popis: | Background Cyclin-dependent kinase (CDK) inhibitors play an important role in regulating cell cycle progression, cell cycle exit and cell differentiation. p27KIP1 (p27), one of the major CDK inhibitors in the retina, has been shown to control the timing of cell cycle exit of retinal progenitors. However, the precise role of this protein in retinal development remains largely unexplored. We thus analyzed p27-deficient mice to characterize the effects of p27 loss on proliferation, differentiation, and survival of retinal cells. Methods Expression of p27 in the developing and mature mouse retina was analyzed by immunohistochemistry using antibodies against p27 and cell type-specific markers. Cell proliferation and differentiation were examined in the wild-type and p27-deficient retinas by immunohistochemistry using various cell cycle and differentiation markers. Results All postmitotic retinal cell types expressed p27 in the mouse retinas. p27 loss caused extension of the period of proliferation in the developing retinas. This extra proliferation was mainly due to ectopic cell cycle reentry of differentiating cells including bipolar cells, Müller glial cells and cones, rather than persistent division of progenitors as previously suggested. Aberrant cell cycle activity of cones was followed by cone death resulting in a significant reduction in cone number in the mature p27-deficient retinas. Conclusions Although expressed in all retinal cell types, p27 is required to maintain the quiescence of specific cell types including bipolar cells, Müller glia, and cones while it is dispensable for preventing cell cycle reentry in other cell types. |
| Databáze: | OpenAIRE |
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