Oncogenic ZEB2/miR-637/HMGA1 signaling axis targeting vimentin promotes the malignant phenotype of glioma
| Autor: | Jie Luo, Ye Song, Anqi Xu, Jie Lin, Cheng Xie, Xi-an Zhang, Tianshi Que, Hao Long, Yu Zeng, Zhiyong Wu, Zhengming Zhan, Shengfeng Ding |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
HMGA1 miR-637 Vimentin migration 03 medical and health sciences 0302 clinical medicine vimentin Downregulation and upregulation Glioma glioma Drug Discovery medicine ZEB2 Zinc finger biology Cell growth Chemistry lcsh:RM1-950 invasion medicine.disease 030104 developmental biology Isocitrate dehydrogenase lcsh:Therapeutics. Pharmacology 030220 oncology & carcinogenesis biology.protein Cancer research Molecular Medicine Homeobox Original Article |
| Zdroj: | Molecular Therapy: Nucleic Acids, Vol 23, Iss, Pp 769-782 (2021) Molecular Therapy. Nucleic Acids |
| DOI: | 10.21203/rs.3.rs-28646/v1 |
| Popis: | Glioma is the most common primary tumor of the central nervous system. We previously confirmed that zinc finger E-box binding homeobox (ZEB) 2 promotes the malignant progression of glioma, while microRNA-637 (miR-637) is associated with favorable prognosis in glioma. This study aimed to investigate the potential interaction between ZEB2 and miR-637 and its downstream signaling pathway in glioma. The results revealed that ZEB2 could directly bind to the E-box elements in the miR-637 promoter and promote cell proliferation, migration, and invasion via miR-637 downregulation. Subsequent screening confirmed that HMGA1 was a direct target of miR-637, while miR-637 could drive the malignant phenotype of glioma by suppressing HMGA1 both in vitro and in vivo. Furthermore, interaction between cytoplasmic HMGA1 and vimentin was observed, and vimentin inhibition could abolish increased migration and invasion induced by HMGA1 overexpression. Both HMGA1 and vimentin were associated with an unfavorable prognosis in glioma. Additionally, upregulated HMGA1 and vimentin were found in isocitrate dehydrogenase (IDH) wild-type and 1p/19q non-codeletion diffusely infiltrating glioma. In conclusion, we identified an oncogenic ZEB2/miR-637/HMGA1 signaling axis targeting vimentin that promotes both migration and invasion in glioma. Graphical Abstract Transcription factor ZEB2 could directly bind to the E-box element in the miR-637 promoter region, leading to upregulation of HMGA1, one of the miR-637 target genes. Moreover, promoted HMGA1 could interact with vimentin and increase both migration and invasion capacity of GBM cells. |
| Databáze: | OpenAIRE |
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