Newcastle disease virus-based MERS-CoV candidate vaccine elicits high-level and lasting neutralizing antibodies in Bactrian camels
| Autor: | Yu Shao, Hui lei Zhang, Zhi yuan Wen, Renqiang Liu, Jin liang Wang, Zhi gao Bu, Jin ling Wang, Jin ying Ge |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Middle East respiratory syndrome coronavirus Agriculture (General) viruses Newcastle disease virus Plant Science medicine.disease_cause Biochemistry Newcastle disease Virus Article S1-972 03 medical and health sciences MERS-CoV Food Animals medicine camels Coronaviridae neutralizing antibodies Pathogen Ecology biology Immunogenicity Embryonated biology.organism_classification Virology 030104 developmental biology Immunization Animal Science and Zoology Agronomy and Crop Science Food Science |
| Zdroj: | Journal of Integrative Agriculture Journal of Integrative Agriculture, Vol 16, Iss 10, Pp 2264-2273 (2017) |
| ISSN: | 2095-3119 |
| Popis: | Middle East respiratory syndrome coronavirus (MERS-CoV), a member of the Coronaviridae family, is the causative pathogen for MERS that is characterized by high fever, pneumonia, acute respiratory distress syndrome (ARDS), as well as extrapulmonary manifestations. Currently, there are no approved treatment regimens or vaccines for MERS. Here, we generated recombinant nonvirulent Newcastle disease virus (NDV) LaSota strain expressing MERS-CoV S protein (designated as rLa-MERS-S), and evaluated its immunogenicity in mice and Bactrian camels. The results revealed that rLa-MERS-S showed similar growth properties to those of LaSota in embryonated chicken eggs, while animal immunization studies showed that rLa-MERS-S induced MERS-CoV neutralizing antibodies in mice and camels. Our findings suggest that recombinant rLa-MERS-S may be a potential MERS-CoV veterinary vaccine candidate for camels and other animals affected by MERS. |
| Databáze: | OpenAIRE |
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