Determination of Phosphorylation Sites in Peptides and Proteins Employing a Volatile Edman Reagent
| Autor: | Jill Meisenhelder, Carl A. Hoeger, Tony Hunter, Wolfgang H. Fischer, Craig Ag |
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| Rok vydání: | 1997 |
| Předmět: |
Electrophoresis
Binding Sites Chromatography Edman degradation Extraction (chemistry) Proteins Biochemistry chemistry.chemical_compound chemistry Isothiocyanates Phosphoserine Reagent Isothiocyanate Humans Phosphorylation Bioorganic chemistry Chromatography Thin Layer Binding site Peptides Phosphorus Radioisotopes Thiocyanates |
| Zdroj: | Journal of Protein Chemistry. 16:329-334 |
| ISSN: | 1573-4943 0277-8033 |
| Popis: | A manual Edman degradation protocol has been developed that allows the identification of phosphorylation sites in 32P-labeled peptides at the subpicomole level. By using both a volatile reagent, trifluoroethyl isothiocyanate, and volatile buffers, extraction steps are rendered unnecessary and cycle times can be reduced to 45 min. The protocol was employed to identify the site of phosphorylation in phosphoserine- and phosphotyrosine-containing peptides. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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