In situ Surface Charge Density Visualization of Self‐assembled DNA Nanostructures after Ion Exchange
| Autor: | Mingdong Dong, Sebastian Amland Skaanvik, Xiaojun Han, Steffan Møller Sønderskov, Lasse Hyldgaard Klausen |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
ADSORPTION PH surface charge density Static Electricity scanning probe microscopy 02 engineering and technology 010402 general chemistry MEMBRANES K+ Microscopy Atomic Force 01 natural sciences Article Scanning probe microscopy Adsorption FUNCTIONALIZED MICA BINDING DNA origami Molecule Physical and Theoretical Chemistry MOLECULE chemistry.chemical_classification Ion exchange STABILITY Biomolecule ORIGAMI NANOSTRUCTURES Charge density Articles DNA 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics 0104 chemical sciences Nanostructures Ion Exchange chemistry SELECTIVITY Chemical physics adsorption Scanning ion-conductance microscopy Nucleic Acid Conformation Aluminum Silicates 0210 nano-technology scanning ion conductance microscopy |
| Zdroj: | Chemphyschem Moller Sonderskov, S, Hyldgaard Klausen, L, Amland Skaanvik, S, Han, X & Dong, M 2020, ' In situ Surface Charge Density Visualization of Self-assembled DNA Nanostructures after Ion Exchange ', ChemPhysChem, vol. 21, no. 13, pp. 1474-1482 . https://doi.org/10.1002/cphc.201901168 ChemPhysChem |
| ISSN: | 1439-7641 1439-4235 |
| DOI: | 10.1002/cphc.201901168 |
| Popis: | The charge density of DNA is a key parameter in strand hybridization and for the interactions occurring between DNA and molecules in biological systems. Due to the intricate structure of DNA, visualization of the surface charge density of DNA nanostructures under physiological conditions was not previously possible. Here, we perform a simultaneous analysis of the topography and surface charge density of DNA nanostructures using atomic force microscopy and scanning ion conductance microscopy. The effect of in situ ion exchange using various alkali metal ions is tested with respect to the adsorption of DNA origami onto mica, and a quantitative study of surface charge density reveals ion exchange phenomena in mica as a key parameter in DNA adsorption. This is important for structure‐function studies of DNA nanostructures. The research provides an efficient approach to study surface charge density of DNA origami nanostructures and other biological molecules at a single molecule level. DNA charge density: The charge density of DNA is a determining factor in strand hybridization and interactions with other molecules in biological systems. Here, the visualization of surface charge density of DNA origami nanostructures is presented under biological relevant conditions using scanning ion conductance microscopy. The findings can be used in the study of surface charge density of DNA origami nanostructure conjugates at the single molecule level. |
| Databáze: | OpenAIRE |
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