Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis
| Autor: | Ken’ichi Ogawa, Yasuaki Shimoishi, Yoshimasa Nakamura, Izumi C. Mori, Shintaro Munemasa, Eiji Okuma, Sarwar Jahan, Megumi Watanabe-Sugimoto, Yoshiyuki Murata, Mohammad Mahbub Islam, Mohammad Anowar Hossain, Daichi Muroyama |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Stomatal conductance
Physiology Glutamate-Cysteine Ligase Arabidopsis Plant Science chemistry.chemical_compound Cytosol Guard cell Dinitrochlorobenzene Arabidopsis thaliana Calcium Signaling Abscisic acid chemistry.chemical_classification Reactive oxygen species biology organic chemicals fungi Cell Membrane Genetic Complementation Test food and beverages Glutathione biology.organism_classification Cell biology Biochemistry chemistry Mutation Plant Stomata Calcium Reactive Oxygen Species Agronomy and Crop Science Abscisic Acid Signal Transduction |
| Zdroj: | Journal of plant physiology. 168(17) |
| ISSN: | 1618-1328 |
| Popis: | We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca(2+)-permeable channel currents by ABA or oscillation of the cytosolic free Ca(2+) concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca(2+) oscillation in ABA signal pathway of Arabidopsis guard cells. |
| Databáze: | OpenAIRE |
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