Identification of Prevotella in Pedal Osteomyelitis of a Diabetic Patient
| Autor: | Barbara J. Dominiak, William C. Oxberry, Patrick C. Chen |
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| Rok vydání: | 2003 |
| Předmět: |
DNA
Bacterial Pathology medicine.medical_specialty Molecular Sequence Data Polymerase Chain Reaction Pathology and Forensic Medicine Prevotella melaninogenica Microbiology Structural Biology Bacteroidaceae Infections medicine Prevotella Humans Dental alveolus DNA Primers Base Sequence biology Osteomyelitis Osteoblast Middle Aged biology.organism_classification medicine.disease Diabetic Foot Resorption Microscopy Electron Diabetes Mellitus Type 1 medicine.anatomical_structure Osteocyte Female Bacteria |
| Zdroj: | Ultrastructural Pathology. 27:271-283 |
| ISSN: | 1521-0758 0191-3123 |
| DOI: | 10.1080/01913120309918 |
| Popis: | Osteomyelitis in a diabetic patient with a nonhealing foot ulcer, multiple medical conditions, and recurrent hospitalization for antibiotic therapy was found to be associated with gram-negative bacteria Prevotella melanginoganica and Prevotella melaninoganica hemagglutinating variant. Those organisms were identified due to the morphologically distinct features in electron microscopy and sequencing of the genes after Polymerase chain reaction amplification from the pathological material. The bacteria invaded the bone and resided in osteocyte, osteoblast, and endothelial cells. The bacteria are usually associated with periodontal plaques, causing inflammation and destruction of gingival tissue and resorption of the alveolar bone. This is the first ultrastructural and molecular study of a diabetic bone lesion with anaerobic bacterial infection. |
| Databáze: | OpenAIRE |
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