Investigation of the potential for sera from cattle persistently infected with bovine viral diarrhea virus to generate false-negative antibody ELISA results in pooled serum from seropositive and seronegative cattle
| Autor: | David Graham, Rónan G. O’Neill, Deirdre King, Tracy A. Clegg |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Diarrhea Viruses
Bovine Viral General Veterinary biology 040301 veterinary sciences Negative antibody 0402 animal and dairy science Persistently infected Enzyme-Linked Immunosorbent Assay 04 agricultural and veterinary sciences Antibodies Viral 040201 dairy & animal science Virology Virus 0403 veterinary science Seroepidemiologic Studies biology.protein Animals Bovine Virus Diarrhea-Mucosal Disease Cattle Viremia Antibody Viral diarrhea Brief Communications False Negative Reactions |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 31(2) |
| ISSN: | 1943-4936 |
| Popis: | We investigated the potential for viremic sera from cattle persistently infected with bovine viral diarrhea virus to create false-negative antibody results when testing pools of 10 sera by indirect or blocking ELISAs. Seronegative viremic sera ( n = 23) were each added to a series of artificially constructed pools containing various percentages (0–90%) of antibody-positive sera, and the resulting pools were assayed for antibody. In all 23 cases, a negative antibody result was obtained in the pool containing no seropositive sera. In contrast, all pools containing ≥10% seropositive serum, representing a single seropositive animal in a pool of 10 samples, returned a positive result in both antibody ELISAs. We concluded that the likelihood of a false-negative antibody result occurring as a result of the presence of serum from a viremic animal was low, and therefore did not preclude the use of pooled sera for serosurveillance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|