Specific Order in the Appearance of Protein Tyrosine Phosphorylation Patterns Is Functionally Coordinated With Dog Sperm Hyperactivation and Capacitation
| Autor: | Anna M. Petrunkina, Anne-Rose Günzel-Apel, Katrin Simon, Edda Töpfer-Petersen |
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| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Urology Endocrinology Diabetes and Metabolism Acrosome reaction Motility Biology chemistry.chemical_compound Dogs Endocrinology Capacitation Internal medicine medicine Animals Phosphorylation Sperm motility Hyperactivation Tyrosine phosphorylation Immunohistochemistry Spermatozoa Sperm Cell biology Reproductive Medicine chemistry Sperm Motility Tyrosine Sperm Capacitation |
| Zdroj: | Journal of Andrology. 24:423-437 |
| ISSN: | 0196-3635 |
| DOI: | 10.1002/j.1939-4640.2003.tb02691.x |
| Popis: | The aims of the present study were to characterize a slow capacitation system that records initial changes in the sperm membrane state, and, using a canine model, to order the specific protein tyrosine phosphorylation signaling in the sequence of capacitational events and to associate them with hyperactivated motility. Dog sperm washed through Percoll were incubated in complete bicarbonate Tyrode medium for 6 hours in 5% CO(2). Capacitation was evaluated using chlortetracycline staining. Tyrosine phosphorylation patterns were assessed by immunocytochemistry. Parallel to this, a computer-assisted motility analysis was performed. Significant changes in the percentage of capacitated and acrosome-reacted cells were first observed after 90 minutes, increasing in a linear manner during further incubation (P |
| Databáze: | OpenAIRE |
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