GM1b and GM1b-GalNAc: major gangliosides of murine-derived macrophage-like WEHI-3 cells
| Autor: | Guiseppe Giordano, Herbert C. Yohe, Walter J. McMurray, Lawrence J. Macala |
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| Rok vydání: | 1992 |
| Předmět: |
Mice
Inbred BALB C Chromatography Gas Ganglioside Chemistry Ratón Macrophages Molecular Sequence Data Biophysics Cell Biology Spectrometry Mass Fast Atom Bombardment Biochemistry Mice chemistry.chemical_compound Immune system Carbohydrate Sequence Cell culture Gangliosides Galactose Tumor Cells Cultured Animals Macrophage Chromatography Thin Layer Myelomonocytic cell Enzymatic degradation |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 1109:210-217 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/0005-2736(92)90085-z |
| Popis: | WEHI-3 cells, derived from a BALB/c mouse, are a myclomonocytic leukemic cell line with macrophage-like properties. We have isolated, purified and characterized the monosialogangliosides from WEHI-3 cells by 1D-HPTLC, 2D-HPTLC, enzymatic degradation, HPTLC-immunostaining, gas liquid chromatography and fast atom bombardment-mass spectrometry (FAB-MS). Quantitative 2D-HPTLC shows two monosialogangliosides are the major components, constituting 77% of the total, with a third monosialoganglioside being 3%. The two major components were identified as (NeuAc)G M1b and (NeuAc)G M1b -GalNAc and the minor component as (NeuAc)G M1b -GalNAc-Gal. The presence of G M1b in this myelomonocytic cell line is consistent with its presence in other murine immune cells and tissues. G M1b -GalNAc and GM 1b -GalNAc-Gal have been reported in T-lineage cells but not is resident or stimulated murine macrophages. Each of these monosialogangliosides belongs to the asialoG M1 synthetic pathway. Preliminary results indicate a disialo member of this pathway. G Dlc , may also be present as a minor component. This ganglioside pathway, containing species which are not sialylated on the internal galactose, appears to be dominant in and may be characteristic of murine immune cells. |
| Databáze: | OpenAIRE |
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