Piscidin-1, an Antimicrobial Peptide from Fish (Hybrid Striped BassMorone saxatilisxM. chrysops), Induces Apoptotic and Necrotic Activity in HT1080 Cells
| Autor: | Yeh Fang Duann, Cheng Hui Lin, Heng Ju Lin, Jheng Fong Lee, Tsui Chin Huang, Sasikala Muthusamy |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Fish Proteins
Cell Antineoplastic Agents Biology Real-Time Polymerase Chain Reaction chemistry.chemical_compound Cell Line Tumor medicine Animals Humans Propidium iodide Cytotoxicity Reverse Transcriptase Polymerase Chain Reaction Acridine orange Hybrid striped bass biology.organism_classification Molecular biology medicine.anatomical_structure Gene Expression Regulation chemistry Cell culture Apoptosis Bass Animal Science and Zoology Ethidium bromide Antimicrobial Cationic Peptides |
| Zdroj: | Zoological Science. 29:327-332 |
| ISSN: | 0289-0003 |
| DOI: | 10.2108/zsj.29.327 |
| Popis: | Piscidin-1, a 22-residue cationic peptide isolated from mast cells of a hybrid striped bass, has potent antimicrobial activities against both gram-positive and -negative bacteria. To date, there is no report of its antitumor activity on any tumor cell lines. In this study, we examined the antitumor activity of a synthetic piscidin-1 peptide against several human cancer cell lines using an MTS assay and soft-agar colony-formation assay. We found that a low dose of piscidin induces both apoptosis and necrosis in HT1080 cells, as shown by annexin-V/propidium iodide and acridine orange/ethidium bromide staining, and triggers a necrotic cell death pathway in a short period with high-dose treatment. The destruction of cell membranes by piscidin-1 was demonstrated by transmission electron microscopy. Furthermore, piscidin-1 also inhibits the migration of HT1080 cells in a dose-dependent manner. This study provides the first evidence of the anticancer activity of the antimicrobial peptide, piscidin-1, with potential implications for the treatment of cancer. |
| Databáze: | OpenAIRE |
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