Characterization of Latilactobacillus curvatus MS2 isolated from Korean traditional fermented seafood and cholesterol reduction effect as synbiotics with isomalto-oligosaccharide in BALB/c mice
| Autor: | Eun-Woo Lee, Tae-Yong Kim, Chan-Hee Kim, Hyun-Tai Lee, Jung-ha Park, Shin-Kwon Kim, Chae Eun Kim, Won Je Jang, Mi-Hyeon Jeon, Su-Jeong Lee, Jong Min Lee |
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| Rok vydání: | 2021 |
| Předmět: |
Male
endocrine system Synbiotics viruses Inulin Biophysics Oligosaccharides Vibrio vulnificus medicine.disease_cause Biochemistry BALB/c chemistry.chemical_compound Mice Republic of Korea medicine Animals Food science Molecular Biology Mice Inbred BALB C biology Chemistry Cholesterol Cell Biology Metabolism biology.organism_classification Antimicrobial Prebiotics Seafood Staphylococcus aureus Lactobacillaceae Fermentation |
| Zdroj: | Biochemical and biophysical research communications. 571 |
| ISSN: | 1090-2104 |
| Popis: | This study investigated the properties of Latilactobacillus curvatus MS2 isolated from Korean traditional fermented seafood as probiotics and the effect of reducing cholesterol as a synbiotic with isomalto-oligosaccharide (IMO) in BALB/c mice. The isolated strain showed high resistance to acids and bile acids and exhibited a high DPPH scavenging capacity of 72.27 ± 0.38 %. In the intestinal adhesion test using HT-29 cells, the adhesion rate of MS2 was 17.10 ± 1.78 %, which was higher than the adhesion rate of the other investigated probiotics. MS2 showed good antimicrobial activity against food-borne pathogens, especially Staphylococcus aureus, S. epidermidis, Escherichia coli, and Vibrio vulnificus. This strain had high availability for IMO among the prebiotics of fructo-oligosaccharide, inulin and IMO. Oral administration of MS2 and IMO to BALB/c mice for 5 weeks resulted in a significant reduction in blood cholesterol levels by regulating liver lipid metabolism. These results suggest that the combination of MS2 and IMO has potential for application in functional foods. |
| Databáze: | OpenAIRE |
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