Resident macrophage-dependent immune cell scaffolds drive anti-bacterial defense in the peritoneal cavity
| Autor: | Alfonso Mora, Margarita Ferriz, Laura H. Villarrubia, Lidia Feo-Lucas, Julieta Alcaín, Carlos Ardavín, Alejandra Gutiérrez-González, Cristina Godio, Natalia Martínez-Puente, Guadalupe Sabio, Adrián Vega-Pérez, María López-Bravo |
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| Rok vydání: | 2021 |
| Předmět: |
Immunology
Biology Peritonitis Article Microbiology Peritoneal cavity Mice Peritoneum medicine Immunology and Allergy Macrophage Animals Peritoneal Cavity Peritoneal Infection Peritoneal fluid Pyroptosis Bacterial Infections Mesothelium B-1 cell Disease Models Animal Infectious Diseases medicine.anatomical_structure Cellular Microenvironment Host-Pathogen Interactions Macrophages Peritoneal Disease Susceptibility Inflammation Mediators Biomarkers |
| Zdroj: | Immunity |
| ISSN: | 1097-4180 |
| Popis: | Summary Peritoneal immune cells reside unanchored within the peritoneal fluid in homeostasis. Here, we examined the mechanisms that control bacterial infection in the peritoneum using a mouse model of abdominal sepsis following intraperitoneal Escherichia coli infection. Whole-mount immunofluorescence and confocal microscopy of the peritoneal wall and omentum revealed that large peritoneal macrophages (LPMs) rapidly cleared bacteria and adhered to the mesothelium, forming multilayered cellular aggregates composed by sequentially recruited LPMs, B1 cells, neutrophils, and monocyte-derived cells (moCs). The formation of resident macrophage aggregates (resMφ-aggregates) required LPMs and thrombin-dependent fibrin polymerization. E. coli infection triggered LPM pyroptosis and release of inflammatory mediators. Resolution of these potentially inflammatory aggregates required LPM-mediated recruitment of moCs, which were essential for fibrinolysis-mediated resMφ-aggregate disaggregation and the prevention of peritoneal overt inflammation. Thus, resMφ-aggregates provide a physical scaffold that enables the efficient control of peritoneal infection, with implications for antimicrobial immunity in other body cavities, such as the pleural cavity or brain ventricles. |
| Databáze: | OpenAIRE |
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