Thyrotropin receptor/thyroglobulin messenger ribonucleic acid in peripheral blood and fine-needle aspiration cytology: diagnostic synergy for detecting thyroid cancer
| Autor: | Mira Milas, S. Sethu, Rosemarie Arciaga, Allan Siperstein, K. V. S. Reddy, Manjula K. Gupta, Ilka Warshawsky, Kristin Wagner |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
Male endocrine system medicine.medical_specialty Adolescent Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Biopsy Fine-Needle Biochemistry Preoperative care Thyroglobulin Endocrinology Internal medicine Cytology Biopsy medicine Humans RNA Messenger Thyroid Neoplasms Thyroid cancer Aged Aged 80 and over medicine.diagnostic_test business.industry Reverse Transcriptase Polymerase Chain Reaction Thyroid disease Biochemistry (medical) Thyroid Cancer Receptors Thyrotropin Middle Aged medicine.disease medicine.anatomical_structure Female business |
| Zdroj: | The Journal of clinical endocrinology and metabolism. 90(4) |
| ISSN: | 0021-972X |
| Popis: | RT-PCR for thyroglobulin (Tg) and TSH receptor (TSHR) mRNA has been used to detect circulating thyroid cancer cells. Little is known, however, regarding the preoperative sensitivity of this test to detect cancer. Seventy-two patients with thyroid disease (36 with malignancy and 36 with benign disease) were evaluated preoperatively. TSHR and Tg mRNA transcripts were detected by RT-PCR assays, previously determined to be specific for cancer cells. There was 100% concordance between TSHR and Tg mRNA RT-PCR results. Of 36 cancer patients, 11 had recurrent disease, and all were positive by RT-PCR. Among 25 patients with no prior thyroid surgery, 18 tested positive preoperatively (sensitivity 72%). Seven of 36 patients with benign disease tested positive (specificity 80%). The overall preoperative diagnostic accuracy was 77%. Preoperative fine-needle aspiration (FNA) biopsy was performed on 46 of 61 patients with no prior thyroid surgery. FNA was diagnostic in 28 (61%) patients. Preoperative cytology was adequate but not diagnostic in 18 (39%) patients. RT-PCR correctly classified 14 of these 18 patients with indeterminate FNA, and the test detected three of four cancer patients as positive (75% sensitive) and 11 of 14 patients (78% specific) with benign disease as negative. The combined diagnostic performance characteristics for RT-PCR and FNA cytology were sensitivity = 95%, specificity = 83%, and diagnostic accuracy = 89%, with positive and negative predictive values of 84 and 95%, respectively. Our results suggest that the molecular detection of circulating thyroid cancer cells by RT-PCR for TSHR/Tg mRNA complements FNA cytology in the preoperative differentiation of benign from malignant thyroid disease and their combined use may save unnecessary surgeries. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|