Virulence determinants of colistin-resistant K. pneumoniae high-risk clones
| Autor: | Mehmet Gönen, Sercin Karahuseyinoglu, Nazli Atac, Onder Ergonul, Ozlem Dogan, Atalay Demiray, Fusun Can, Cansel Vatansever, Bilge Kaan Kilicoglu, Ozgur Albayrak, Ozgun Ekin Sahin |
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| Přispěvatelé: | Ergönül, Mehmet Önder (ORCID 0000-0003-1935-9235 & YÖK ID 110398), Gönen, Mehmet (ORCID 0000-0002-2483-075X & YÖK ID 237468), Can, Füsun (ORCID 0000-0001-9387-2526 & YÖK ID 103165), Doğan, Özlem (ORCID 0000-0002-6505-4582 & YÖK ID 170418), Vatansever, Cansel, Ataç, Nazlı, Albayrak, Özgür, Karahüseyinoğlu, Serçin (ORCID 0000-0001-5531-2587 & YÖK ID 110772), Şahin, Özgün Ekin, Kılıçoğlu, Bilge Kaan, Demiray, Atalay, School of Medicine, Graduate School of Health Sciences, College of Engineering, Department of Industrial Engineering |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
QH301-705.5 Operon Phagocytosis 030106 microbiology Virulence Biology Life sciences Biomedicine Yersiniabactin General Biochemistry Genetics and Molecular Biology Microbiology 03 medical and health sciences chemistry.chemical_compound Biosynthesis medicine Biology (General) Gene General Immunology and Microbiology iron uptake K. pneumoniae K pneumoniae phagocytosis net formation Colistin resistance Pneumoniae Iron uptake Net formation 030104 developmental biology colistin resistance chemistry Colistin General Agricultural and Biological Sciences medicine.drug |
| Zdroj: | Biology Biology, Vol 10, Iss 436, p 436 (2021) Volume 10 Issue 5 |
| Popis: | We proposed the hypothesis that high-risk clones of colistin-resistant K. pneumoniae (ColR-Kp) possesses a high number of virulence factors and has enhanced survival capacity against the neutrophil activity. We studied virulence genes of ColR-Kp isolates and neutrophil response in 142 patients with invasive ColR-Kp infections. The ST101 and ST395 ColR-Kp infections had higher 30-day mortality (58%, p = 0.005 and 75%, p = 0.003). The presence of yersiniabactin biosynthesis gene (ybtS) and ferric uptake operon associated gene (kfu) were significantly higher in ST101 (99%, p ≤ 0.001) and ST395 (94%, p < 0.012). Being in ICU (OR: 7.9 CI: 1.43–55.98 p = 0.024), kfu (OR:27.0 CI: 5.67–179.65 p < 0.001) and ST101 (OR: 17.2 CI: 2.45–350.40 p = 0.01) were found to be predictors of 30-day mortality. Even the neutrophil uptake of kfu+-ybtS+ ColR-Kp was significantly higher than kfu--ybtS- ColR-Kp (phagocytosis rate: 78% vs. 65%, p < 0.001), and the kfu+-ybtS+ ColR-Kp survived more than kfu--ybtS- ColR-Kp (median survival index: 7.90 vs. 4.22 p = 0.001). The kfu+-ybtS+ ColR-Kp stimulated excessive NET formation. Iron uptake systems in high-risk clones of colistin-resistant K. pneumoniae enhance the success of survival against the neutrophil phagocytic defense and stimulate excessive NET formation. The drugs targeted to iron uptake systems would be a promising approach for the treatment of colistin-resistant high-risk clones of K. pneumoniae infections. |
| Databáze: | OpenAIRE |
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